In vitro Nematicidal Activity of Ten Plant Extracts Against Juveniles of Meloidogyne incognita | ||||
Egyptian Journal of Agronematology | ||||
Article 7, Volume 14, Issue 1, January 2015, Page 78-90 PDF (253.22 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejaj.2015.60403 | ||||
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Authors | ||||
M. Saeed1; G. Awadh1; M. Al-Thobhani2 | ||||
1Department of Plant Protection, Faculty of Agriculture, Sana'a University, Yemen. | ||||
2Department of Horticulture and forestry, Faculty of Agriculture, Sana'a University, Yemen. | ||||
Abstract | ||||
The nematicidal potential of aqueous leaf extracts of ten plant species distributed in agricultural lands in Yemen were assessed against second stage juveniles of Meloidogyne incognita in laboratory. The juveniles were exposed to 6, 12, 24 and 48 hrs in three concentrations (12.5, 25 and 50%) of leaf extracts. Leaf extracts of Datura stramonium, Peganum harmala, Datura innoxia, Argemone mexicana and Nicotiana glauca were effective in causing juvenile mortality, while leaf extracts of the other five plant species, Azadirachta indica, Catha edulis, Solanum incanum, Tagetes minuta and Withania somnifera showed no or little nematode mortality (< 30 %) even at 50 % concentration and 48 hrs exposure time. The juveniles mortality was increased with increase of concentration and exposure time. Hundred percent juveniles mortality was observed at the modest concentration (25%) of leaf extracts of D. stramonium and D. innoxia after 24 and 48hrs, respectively. Whereas 100 % mortality of juveniles was found at highest concentration (50%) in leaf extract of D. stramonium after 12 hrs and in leaf extracts of D. innoxia, P. harmala and N. glauca after 24 hrs or after 48 hrs in case of leaf extracts of A. mexicana. Leaf extract of D. stramonium achieved the highest mortality percentage at different concentrations and was significantly (p≤0.05) superior over the rest of the extracts applied over time. | ||||
Keywords | ||||
Meloidogyne incognita; Nematicidal activity; Juveniles mortality; Leaf extracts | ||||
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