The Use of Serum Survivin and AFP-L3 and their Combination in Improving Hepatocellular Carcinoma Diagnosis Especially in Patients with Low Serum AFP | ||||
| The Medical Journal of Cairo University | ||||
| Article 11, Volume 86, December, December 2018, Page 3467-3472 PDF (450.79 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2018.60585 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| MAHMOUD MOAWAD, Ph.D.; ANWAR MOHAMED, M.D. | ||||
| The Departments of Surgical Pathology, National Cancer Institute, Cairo University* and Hepatology, National Liver Institute, Menoufia University**, Menoufia, Egypt | ||||
| Abstract | ||||
| Abstract Background: Hepatocellular carcinoma (HCC) is respon-sible for about 4.7% of chronic liver disease in Egypt. Survivin is an essential element in apoptosis inhibition. Survivin is present in fetal and malignant adult tissue and is absent in non malignant tissues. A diagnostic and prognostic HCC marker is the alpha-fetoprotein (AFP) binding to Lens culinaris agglutinin (AFP-L3%) percentage. There is a need for HCC marker that is better than AFP especially in those low AFP. Aim of Study: To detect the role of determination of serum Survivin gene, AFP-L3% and their combination in HCC diagnosis. Subjects and Methods: Fifty HCC patients and 20 healthy control persons were subjected for measurement of serum AFP, survivin gene and AFP-L3%. Reverse transcription-polymerase chain reaction (RT-PCR) was used for detection of serum survivin gene. AFP-L3% was measured by a liquid-phase binding assay on the Wako LiBASys clinical auto analyzer. Results: Serum Survivin gene was positive in 27 (54%) HCC cases and negative in 23 (46%) cases. AFP-L3% was detected in 36 (72%) HCC cases. The combined use of serum servivin gene and AFP-L3% were positive in 43 (86%) of HCC cases. In 22 HCC cases with serum AFP below 200 ng/ml serum servivin gene was detected in 9 (40.9%) cases but AFP-L3% was positive in 13 (59.9%) cases and the combined use of positive serum servivin and AFP-L3 were present in 18 cases (81.18%). Conclusions: The combined use of serum survivin gene and AFP-L3% could have a valuable diagnostic role in HCC especially in those with low AFP. | ||||
| Keywords | ||||
| HCC – Survivin – AFP – AFP-L3 – Tumor markers | ||||
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