EFFECT OF CYTOCHROME P450 1A1 (CYP1A1)POLYMORPHISM ON ASPHALT EXPOSURE - RELATED BIO-MARKERS | ||||
| Egyptian Journal of Occupational Medicine | ||||
| Article 3, Volume 31, Issue 1, January 2007, Page 29-49 PDF (103.01 K) | ||||
| Document Type: Study paper | ||||
| DOI: 10.21608/ejom.2007.613 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Farahat A1; Helal F.1; Abdel N.1; Rashed A.2 | ||||
| 1Department of Occupational Medicine & Industrial Diseases | ||||
| 2Department of Biochemistry,Faculty Of Medicine . Cairo University | ||||
| Abstract | ||||
| Asphalt fumes contain polycyclic aromatic compounds (PAC). There is a possibility of long-term health effects following chronic exposure by inhalation or skin contamination in asphalt road pavers. Objective: This study aimed at exploring the relationship between urinary 1-OH pyrene as a marker of internal dose of PAHs exposure and serum P53 protein as a response marker with the genetic polymorphism of CYP1A1 as a susceptibility marker among asphalt exposed workers. Methods: This work included 70 male individuals who were divided into an asphalt exposed group (n=43) and a matched control group (n=27). The exposed group was further subdivided into a group of paving and mixing (n=24) and a group of roller and vehicle drivers (n=19). Every participant was subjected to polymerase chain reaction (PCR) technique to detect the different genotypes of CYP1A1 gene and the expression of the p53 gene mutation. Besides, estimation of urinary 1-hydroxy pyrene (1-OHP) and serum level of mutant p53 protein was done as a PAHs exposure and response biomarkers, respectively. Results: CYP1A1*2A (Valine / Valine) was found to be the most prevalent CYP 1A1 genotype among both exposed and control groups. It was associated | ||||
| Keywords | ||||
| Asphalt-PAHs-CYP1A1- CYP1A1*2A-CYP1A1*1- CYP1A1*2CPolymorphism-P53 gene mutation- Urinary 1-OH Pyrene | ||||
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