Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

El-Dakhly, A. T.; Youssef, M. M.; Tammam, Abeer A.; Moedham, Namaa A.

doi:10.21608/javs.2016.62123

	Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
Journal of Applied Veterinary Sciences
Article 10, Volume 1, Issue 1, October 2016, Page 69-72 PDF (418.32 K)
Document Type: Original Article
DOI: 10.21608/javs.2016.62123
View on SCiNiTO
Authors
A. T. El-Dakhly ¹; M. M. Youssef¹; Abeer A. Tammam¹; Namaa A. Moedham²
¹Rinder Pest Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
²Pox Vaccines Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
Abstract
This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9^th day post inoculation to reach the best titre 6 log10 TCID50 /ml.
Keywords
VERO cell; Peste Des Petits Ruminants; cell growth


References
ADOMBI, C.M ; LELENTA, M; LAMIEN, C.E; SHAMAKI, D; KOFFI, Y.M; TRAORE, A; SILBER, R; COUACY-HYMANN ,E; BODJO, S.C; DJAMAN, J.A; LUCKINS, A.G AND DIALLO A. 2011. Monkey CV1 cell line expressing the sheep–goat SLAM protein: a highly sensitive cell line for the isolation of peste des petits ruminants virus from pathological specimens. J. Virol. Methods; 173:306–313. AHMED, M.M. 1990. Susceptibility of different tissue culture cells to virus of pest des petites ruminants. M. Vet. Sci. Thesis (Microbiology), Fac. Vet. Med. Alex University. ASIM, M; A. RASHID; A. H. CHAUDHARY AND M. S. NOOR 2009. Production of homologous live attenuated cell culture vaccine for the control of peste des petits ruminants in small ruminants. Pakistan Vet. J., 29(2): 72-74. BERRY, J.M; BARBABE, N; COOMBS, K.M. AND BUTLER, M. 1999. Production of reovirus type 1 and type 3 from Vero cells grown on solid and macroporous microcarriers. Biotechnology and Bioengineering, 62 (1): 12-19. DIALLO, A; BARRETT, T; BARBRON, M; SHAILA, M.S AND TAYLOR, W.P 1989A. Differentiation of rinderpest and peste des petits ruminants’ viruses using specific cDNA clones. J Virol Methods. 23:127–136. DIALLO, A; TAYLOR, W. P; LEFEVRE, P.C AND PROVOST, A 1989B. Attenuation dʼune souche de virus de la Peste Des Petits Ruminants: candidat pour un vaccine homologue vivant. Rev.Elev. Med. Vet. Pays Trop; 42, pp .311-319. JOHN, K; CHANDRAN, N. D. J; RAMASWAMY, V; BALACHANDRAN, C AND KIRUBAHARAN, J .J 2006. Adaptation of PPR vaccine virus in BHK₂₁ monolayer culture Indian Vet. J.1143-1146. LEFEVRE, P.C AND DIALLO, A 1990. Peste des petits ruminants. Rev. Sci. tech. Off. Int. Epiz, 9, 951–965. MARINER, J.C; HOUSE, J.A; SOLLOD, A.E; STEM, C; ENDE, M.V.D AND MEBUS C.A 1990. Comparison of the effect of various chemical stabilizers and lyophelization cycles on the thermo stability of a vero cell-adapted rinderpest vaccine. Vet Microbiol. 21:195–209. MAYLING ALVAREZ; ROSMARI RODRIGUEZ-ROCHE; LÌDICE BERNARDO; LUIS MORIER AND MARIA, G. GUZMAN 2005. Improved Dengue Virus Plaque Formation onBHK21 and LLCMK2 Cells: Evaluation of Some Factors. Dengue Bulletin ñ Vol 29. MOHAN, M 2004. MVSc Thesis submitted to the Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007. MOHAN, M; KOTEESWARAN, A AND CHANDRAN, N.D.J 2009. High titre peste des petits ruminants (ppr) vaccine virus. Tamilnadu J. Veterinary & Animal Sciences 5 (4) 149-154. MOSS W.J AND GRIFFIN D.E 2006. Global measles elimination. Nat. Rev. Microbiol. 4:900–908. (OIE), 2006 World Organization for Animal Health, Office International des Epizooties (OIE)Manual of standards for diagnostic tests and vaccines, 6^th Ed. OIE, Paris, pp1036-1046. OSMAN, O.A ; SABER, M.S.; EL-SENOSY, A.A.; ABBAS, A.M.; MOUAZ, M.A.; HUSSEIN, A.H. AND KHODEIR, M.H. 1994. Detection of PPR virus in Vero cells using fluorescent antibody technique. Zag. Vet. Med. J. 22 (3): 111-119 PAILLET, C; FORNO, G; KRATJE, R AND ETCHEVERRIGARAY, M 2009. Suspension-Vero cell cultures as a platform for viral vaccine production.Vaccine. 30; 27(46):6464-7. RASHWAN, S.M; H.N. AL-KHALAF; M.I. AL-HAMMAD; A.H. AZAB; M.H. MOUSTAFA AND A. DIALLO 2000. Production and Efficacy of Peste Des Petits Ruminants Vaccine 75/1 in the Kingdom of Saudi Arabia .J. Appl. Anim. Res. 18: 225-231 REED, I.J AND MUENCH, H.A. A 1938. .Simple method of estimating fifty per cent endpoints Am. J. Hyd; 27: 493-497. ROSSITER, P.B; JESSETT, D.M. AND TAYLOR, W.P (1985). Micro neutralization systems for use with different strains of peste des petits ruminant’s virus and rinderpest virus. Tropical Animal Health and Production, 17, 75–81. ROSSITER, P.B AND WARDLEY, R.C (1985). The differential growth of virulent and avirulent strains of rinderpest virus in bovine lymphocytes and macrophages. Journal of General Virology, 66, 969–975. SEKI, F; ONO, N; YAMAGUCHI, R AND YANAGI, Y 2003. Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD150) and their adaptability to marmoset B95a cells. J. Virol.; 77:9943–9950. VAN REGENMORTEL, M.H; MAYO, M.A; FAUQUET, C.M AND MANILOFF, J 2000.Virus nomenclature: consensus versus chaos. Arch Virol. 145:2227–2232. WESTWOOD, J. C. N; APPLEYARD, G; TAYLOR-ROBINSON, D AND ZWARTOUW, H. T. 1960. The production of high titre poliovirus in concentrated suspensions of tissue culture cells. British Journal of Experimental Pathology 4 I, l05. WHO, 1970. Normes No 19 pour les substances biologiques. Comite OMS d'experts de la standardisation biologique. 22e rapport, annexe 1 OMS Geneve 1970 Serie de rapports techniques: No 444. KOCK, R.A., MIHOK, S.R.O., WAMBUA, J., MWANZIA, J., SAIGAWA, K. 1999. Effects of translocation on hematologic parameters of free-ranging black rhinoceros (Diceros bicornis michaeli) in Kenya. Journal of Zoo and Wildlife Medicine 30: 389-396. ‏MARCO, I., VIÑAS, L., VELARDE, R., PASTOR, J. AND LAVÍN, S. 1997. Effects of capture and transport on blood parameters in freeranging mouflon 12-751. SHALAH, M.R.1983. The role of camels in overcoming world meat shortage. Egyptian J. Vet. Sci. 1983, 20:101–110. Cited by Nazifi,S.et al (2009). SUNDARESAN, G., SUTHANTHIRARAJAN, N., & NAMASIVAYAM, A. 1990. Certain immunological parameters in subacute cold stress. Ind. J. Physiol, 34, 51-60. WARRIS, P.D. 1998. The welfare of slaughter pigs during transport. Anim Welfare 7: 365-381. YAGIL,R.1982. Camels and Camel Milk. FAO Animal Production and Health. Publications Division, Food and Agriculture Organization of the United Nations. Via delleTerme di Caracalla, 00100 Rome, Italy,26. YOUSIF,O.K,BABIKER,S.A.1989. The desert camel as meat animals. MeatSci. 26: 245–254. Cited by Nazifi,S.et al (2009).
Statistics Article View: 363 PDF Download: 545