Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 1, Volume 2, Issue 1, October 2017, Page 1-8 PDF (648.17 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2017.62129 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ramadan A.1; Nehal A. Afifi1; Nemat Z. Yassin2; Rehab F. Abdel-Rahman2; Sahar S. Abd El-Rahman3; Hany M. Fayed* 2 | ||||
| 1Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||||
| 2Pharmacology Department, Medical Division, National Research Centre, Giza, Egypt. | ||||
| 3Pathology Department, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||||
| Abstract | ||||
| To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks. Group II served as (TAA group). Groups III and IV (Treatment groups): rats were given tranilast for 6 weeks after TAA discontinuation. Liver osteopontin (OPN), transforming growth factor-β (TGF-β1), tumor necrosis factor alpha (TNF-α), alpha-smooth muscle actin (ɑ-SMA)), reduced glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. Additionally, expression of α-smooth muscle actin (SMA) and caspase (Cas)-3 were assigned immunohistochemically. Treatment with tranilast prevented the development of hepatic fibrosis and the activation of stellate cells, and down-regulated the expression of genes for osteopontin and osteopontin-target molecules, including TGF-β and TNF-α and α-SMA.Tranilast significantly decreased MDA and increased levels of GSH, and SOD. Our findings suggest that targeting osteopontin with tranilast represents a new mode of therapy for liver fibrosis. | ||||
| Keywords | ||||
| Osteopontin; Liver fibrosis; apoptosis; antioxidant | ||||
| References | ||||
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