Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers
Khafagy, A., Eid, H., Youssif, F., Eid, N. (2017). Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers. EKB Journal Management System, 22(2), 107-120. doi: 10.21608/scvmj.2017.62157
Ahmed Khafagy; Hamza Eid; Fatma Youssif; Nour El Hoda Eid. "Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers". EKB Journal Management System, 22, 2, 2017, 107-120. doi: 10.21608/scvmj.2017.62157
Khafagy, A., Eid, H., Youssif, F., Eid, N. (2017). 'Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers', EKB Journal Management System, 22(2), pp. 107-120. doi: 10.21608/scvmj.2017.62157
Khafagy, A., Eid, H., Youssif, F., Eid, N. Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers. EKB Journal Management System, 2017; 22(2): 107-120. doi: 10.21608/scvmj.2017.62157

Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers

Suez Canal Veterinary Medical Journal. SCVMJ
Article 10, Volume 22, Issue 2, December 2017, Page 107-120  XML PDF (644.81 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2017.62157
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Authors
Ahmed Khafagy1; Hamza Eid1; Fatma Youssif2; Nour El Hoda Eid* 3
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University
2Animal Health Research Institute, Ismailia
3North Sinai Department of Veterinary Medicine
Abstract
In the present study (10) E.coli strains previously isolated from (450) raw milk samples of different sources were analyzed for the determination of their antibiogram and screened for the presence of alkaline phosphatase gene (phoA)  and virulence markers for Shiga toxin producing E.coli (STEC); stx1, stx2, and  the occurrence of antibiotic resistance genes; nonspecific TEM β-Lactamase (blaTEM),Tetracycline A (tetA), which were investigated by Polymerase chain reaction. Ten E.coli strains were tested for the antibiotic resistance by using the AST card of the VITEK2. The antibiotic sensitivity test showed a high resistance towards Ampicillin (100%), Tetracycline (80%), Piperacillin(50%) and Chloramphenicol, Cephaexin and Sulfamethaxole-Trimethoprim (40%) for each and 30% of the isolates showed intermediate resistance toward Amoxicillin and Clavulinic acid. The polymerase chain reaction (PCR)was used to detect the genes encoding for alkaline phosphatase (phoA) , Shiga toxin type 1(stx1),Shiga toxin type 2( stx2) , Nonspecific TEM β-Lactamase (blaTEM),Tetracycline A (tetA).The results showed that all the investigated E.coli strains(n=10) harbored the alkaline phosphatase gene(phoA),100%.The Shiga toxin type one encoding gene(stx2) was found in one E.coli strain (O158), while none of E.coli strains has the Shiga toxin type 1 gene (stx1).One hundred percentage of the screened E.coli strains carried the blaTEM gene, while 80% of the strains harbored the tetA gene.
 
Keywords
E.coli; Antibiogram; phoAgene; Virulence genes; resistance genes
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