THE POSSIBLE PROTECTIVE ROLE OF NIGELLA SATIVA OIL AND VITAMIN E AGAINST RADIATION IN ADULT MALE ALBINO RATS | ||||
| Egyptian Society of Clinical Toxicology Journal | ||||
| Article 1, Volume 2, Issue 1, June 2014, Page 1-16 PDF (399.03 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/esctj.2014.62373 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Marwa Abaza  1; Iman Elkhishin1; Ghada Elmesallamy2; Mona Elghazaly3
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| 1Forensic Medicine and Clinical Toxicology, Department, Faculty of Medicine, Zagazig University, Egypt | ||||
| 2Forensic Medicine and Clinical Toxicology, Department, Faculty of Medicine, Zagazig University, Egypt | ||||
| 3National Centre for Radiation Research and Technology, Egypt | ||||
| Abstract | ||||
| Background: The potential application of ionizing radiation in medical practice and also potential accidental exposure to radiation, enhanced the development of effective radio modifier. The aim of this work was to evaluate the possible protective role of individual & combined administration of Nigella sativa(NS) and vitamin E against the toxic effects of single 6 Gray (Gy) dose of gamma radiation on adult male albino rats Material and methods: ninety adult male albino rats. They were divided into 9 groups each of 10 rats: Group 1: (-ve control), group II (+ve control) , group III (Irradiated group) each rat was exposed to a single dose of radiation 6 Gy , group IV: each rat was gavaged orally with 0.6 ml NS dissolved in one ml distilled water once daily 5days / week, group V: each rat was gavaged 100 mg\ kg vitamin E dissolved in one ml corn oil once daily 5days / week, group VI: each rat received both vitamin E and NS orally in the previous doses once daily for 5 days / week, group VII: each rat received NS oil for 5days/week daily at the 3rd day each rat was exposed to a single radiation in the previous doses, group VIII: each rat received vitamin E daily for 5days/week at the 3rd day each rat was exposed to a single radiation in the previous doses, group VIIII: each rat received both vitamin E and NS daily for 5days/week at the 3rd day each rat was exposed to a single radiation in the previous doses. After 15 days from last exposure, rats of each subgroup were submitted to estimate the complete blood count (CBC) and oxidative stress parameters .then the rats were sacrificed .The spleen and bone marrow in adult male albino rats were dissected and subjected to histopathological examination. Results: There was a significant deterioration in all measured parameters in irradiated group. Upon supplementation with individual and combined NS and vitamin E before radiation, there were significant improvements in these measured parameters when compared with irradiated group. The histopathological changes in the spleen were in the form of white pulp depletion and multiple areas of hemorrhage. It also was found bone marrow affection denoted by marked depletion of bone marrow tissue. Upon administration of individual and combined NS and vitamin E to irradiated rats an improvement was observed in the form of regression of the changes that occurred after radiation exposure. Biochemical and histopathological improvement in combined NS and vitamin E administration better than individual NS and vitamin E administration was noticed. Recommendation: More efforts are needed to explore efficient natural combined radio protectors to limit deleterious effect of radiation. | ||||
| Keywords | ||||
| Nigella sativa oil; Vitamin E; Radiation; acute radiation; individual combined | ||||
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