Multiplex PCR of Some Antibiotic Resistance Genes of Methicillin Resistant Staphylococcus Aureus (MRSA) Isolated from Infected Cat Fish (Clarias garipeneaus). | ||||
Suez Canal Veterinary Medical Journal. SCVMJ | ||||
Article 3, Volume 22, Issue 1, June 2017, Page 31-42 PDF (583.95 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/scvmj.2017.62376 | ||||
View on SCiNiTO | ||||
Authors | ||||
Ahmed Khafagy1; R. El Gamal2; Hala F.2; Samaa A.* 3 | ||||
1Dept. of Microbiology Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt. | ||||
2Central laboratory for Aquaculture Research, Agriculture Research Center, Egypt | ||||
3Department of Microbiology, Faculty of Vet. Medicine, Suez Canal University, Ismailia, Egypt. | ||||
Abstract | ||||
This study was designed to detect some antibiotic resistance genes of S.aureus by using PCR. A total of 150 Cat fish (Clarias garipeneaus) with a body weight ranged from200 – 250g. suffered from ulcerated skin and erosion. S. aureus were isolated in percent of 100% from infected fish by using Baird parker as selective media. By using multiplex PCR for investigation some antibiotic resistant genes, as erm A gen, aac (6)-aph (2) genes , van A gen and mec A gene. All isolated S. aureus were sensitive to Amikacin, Amoxicillin, Ampicillin and Ciprofloxacin while resistant to Gentamicin, Methicillin and Neomycin. | ||||
Keywords | ||||
PCR; Antibiotic resistance genes; Staphylococcus aureus; Cat Fish | ||||
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