Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 1, Volume 4, Issue 1, April 2019, Page 1-4 PDF (267.17 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2019.62668 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Hanan M. Ibrahim; Hanan A. Ahmed; Nourhan Nagy  ; Gina M. Mohamed; Shafai S.M; Aly A.M
| ||||
| Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)El-Seka El-Baida, Abbasia, Cairo, Egypt | ||||
| Abstract | ||||
| A total number of 27 vaccine vials including 3 batches of 3 different types of live attenuated poultry viral vaccines (tissue culture Gumboro vaccine, egg adapted colored ILT vaccine and egg adapted non colored ILT vaccine) were experimentally contaminated with 1,5,10 CFU of Salmonella typhimurium reference strain/dose then subjected for sterility testing as referenced in OIE. Further dilution was carried out of vaccines showing negative results. The results of statical analysis showed that there is a significant difference between direct inoculation of the vaccines and their dilutions. It could be concluded that the applied testing method is appropriate for testing sterility of living attenuated poultry viral vaccines but testing of some of such vaccines need further dilutions to obtain accurate results. | ||||
| Keywords | ||||
| Bacterial contamination; Detection limits; live attenuated vaccines | ||||
| References | ||||
|
ANNEX 1. 2017. Manufacture of sterile Medical Prroducts, European Commission December 2017.
CODE OF FEDERAL REGULATION "CFR". 2015. Title 9. Animals and Animal Products.
EXCEL KONTROL (EN) 2008. NEW CONTROL X-CHART FOR A NEW CONTROL CHART (Excelcontrol@gmail.com).
EXCEL KONTROL VERSION 2.1. 2008. Rules for out of control
ISO8402. 1994. Quality management and quality assurance – vocabulary Rev. Sci. Tec. Off. Epiz., 20
ISO/TS 11133-1. 2014. General guideline on quality assurance for preparation of culture media in the laboratory.
ISO TS 34SC9W03 / 16140. 2017. Microbiology of the food chain - method validation- part 3protocol for the verification of reference and validated alternative methods implemented in single laboratory Published by the Office of the Federal Register. National Archives and Records Administration
OIE 2016. Infection disease in OIE quality standard and guidelines for veterinary laboratories (world organization for health).
LEE, J.Y. 1990. Investigating Sterility Test Failure. Pharm. Technol., 38-43.
MARANGON S. AND BUSANI L. 2005. The use of vaccination in poultry production. Rev. Sci. Tec. Off. Epiz., 20 Reynolds, J.: American Society for Microbiology, 6, 26 (1), 265-274.
REYNOLDS,J. 2005. American Society for Microbiology, 6, 26 (1), 265-274.
ROSS TECH NOTE 2008. Drinking Water Vaccination- a few simple roles. Africa Ferndandez, Company Veterinarian, Avigen, September 2008.
| ||||
|
Statistics Article View: 557 PDF Download: 446 |
||||
