In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles | ||||
Egyptian Journal of Cancer and Biomedical Research | ||||
Article 6, Volume 3, Issue 2 - Serial Number 4, August 2019, Page 27-38 PDF (384.56 K) | ||||
Document Type: Original Article | ||||
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Authors | ||||
Yousry E El-Bolkiny 1; El-Refaie Kenawy2; Ibrahim Al-Shourbagy3; Mohmed Basyony3; Reda Salama3; Abdelhalim Alkhazendar 4 | ||||
1Department of Zoology, Faculty of Science, Tanta University, Egypt | ||||
2Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt. | ||||
3Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt. | ||||
4Zoology Department, Faculty of Science, Tanta University | ||||
Abstract | ||||
Biologically active agents show several biomedical applications. Non-chitosan loaded diethyldithiocarbamate (DDC) was previously found to have anti-schistosomal activity. The present work aimed to prepare DDC-Chitosan-Na-nanoparticles (DDC-Chitosan-Na-NPs), and to investigate it’s in vitro anti-schistosomal activity with slow release approach and as well as its mortality % against both concentrations and exposure time. NPs were prepared by suspension cross linking method, in which 0.16 g chitosan were dissolved in 16 ml 2% acetic acid solution (ratio 1% wt/v.). Then after, 0.1g DDC-Na salt was added to the chitosan solution and 3.2 ml of glutaraldehyde as a cross linking agent was added into the mixture dropwise. NPs were washed several times with ethanol 70%, dried under vacuum, prepared for chemical characterization and biological assessment. Our data showed that the slow release of DDC-chitosan-NPs resulted in mortality effects on Schistosomal worms in vitro when applied with small dose for longer time. These data compared to those of prazoquantil (PZQ) as a reference drug showed that administration of DDC loaded with chitosan-NPs was more effective on the mortality of Schistosoma worms. In conclusion, DDC-Chitosan-NPs have lethal effects on S. mansoni worms in vitro by a unique mode of action in a way of concentration and exposure time dependent. Also we recommend that the development of DDC as a local, cheaper and more effective agent against S. mansoni worms. | ||||
Keywords | ||||
Metformin; Hamsters; Pancreatic cancer; N-Nitrosobis (2-oxopropyl) amine | ||||
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