The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells
El-jaffry, S., Fehalbum, I., A. A, H., Hussein, M. (2013). The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells. EKB Journal Management System, 9(1), 1-5. doi: 10.21608/blj.2013.63403
Salem El-jaffry; I. Fehalbum; Haytham. A. A; Mohamed M.A. Hussein. "The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells". EKB Journal Management System, 9, 1, 2013, 1-5. doi: 10.21608/blj.2013.63403
El-jaffry, S., Fehalbum, I., A. A, H., Hussein, M. (2013). 'The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells', EKB Journal Management System, 9(1), pp. 1-5. doi: 10.21608/blj.2013.63403
El-jaffry, S., Fehalbum, I., A. A, H., Hussein, M. The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells. EKB Journal Management System, 2013; 9(1): 1-5. doi: 10.21608/blj.2013.63403

The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells

Biochemistry Letters
Article 1, Volume 9, Issue 1, 2013, Page 1-5  XML PDF (915.9 K)
Document Type: Original Article
DOI: 10.21608/blj.2013.63403
View on SCiNiTO View on SCiNiTO
Authors
Salem El-jaffry1; I. Fehalbum1; Haytham. A. A2; Mohamed M.A. Hussein2
1Department of Biochemistry, Faculty of Medicine, Tripoli, Libya
2Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt.
Abstract
 




 A series of experiments was designed to investigate the activity of the SV40 early promoter and enhancer in rat pituitary tumor cells in culture but still yet not clear so, a series of enhancer promoter-reporter plasmids were constructed to study the SV40 activity in the rat pituitary tumor cell lines (GH3 and GC) then sub-cloned into pSV2(enhancer-minus)-CAT, then purified in gel and ligated into pRSV(enhancer-minus)-CAT (Sph1 restricted) then transformed into E.Coli then make restriction analysis. From the results we observed that SV40 early promoter can be activated by heterogeneous enhancers in pituitary tumor cells. 
Statistics
Article View: 110
PDF Download: 154