Protective Effects Of Solanium Nigrum Methanolic Extract Against Isoniazid/Rifampicin Induced Hepatotoxicity In Rats | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 8, Volume 4, Issue 2, October 2019, Page 52-61 PDF (748.71 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2019.63436 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Abdel Hameed M. Fayed  1; Abeer A.A. Salama1; Ismaiel E Ismaiel1; Taha A. Attia2; Elbatran Seham A.1; Azza Hassan3
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| 1Pharmacology Department National Research Centre, Giza, Egypt | ||||
| 2Pharmacology Department, Faculty of Vet. Med., El-Sadat University, Minoufiya, Egypt | ||||
| 3Pathology Department, Faculty of Vet. Med., Cairo University, Giza, Egypt | ||||
| Abstract | ||||
| Drug-induced liver injury (DILI) is the major health problem that resulted from uses of many drugs especially from serious adverse effect of antituberculosis (ATT) drugs. forty eight rats (200-250g) were allocated into six groups (8 rats in each group), and treated as follow: group I: received normal saline orally; group II: received INH/RFP (50 mg/Kg/day of each) for 28 days orally; group III: received Silymarin (50 mg/kg/day) + INH/RFP for 28 days orally; group IV: received SNME(250 mg/Kg/day) + INH/RFP for 28 days orally; group V: received SNME (500 mg/Kg/day) + INH/RFP for 28 days orally; group VI, received SNME (1000 mg/Kg/day) + INH/RFP for 28 days orally. Co-administration of herbal plant (SNME) with INH/RFP reduced elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and (bilirubin total &direct) levels but also decreased the elevated malondialdehyde (MDA) & tumor necrosis factor-α (TNF-α) contents in liver homogenate. Moreover, co-administration of herbal plant (SNME) with INH/RFP increased glutathione peroxidase (GPX) & adiponectin activity. Solanum nigrum administration to rats, in present study, led to a remarkable alteration of histological changes observed in INH/RFP group. It is concluded that Solanum nigrum might be considered as adjuvant drug in treatment of liver disorder and/or as hepatoprotective therapy with anti-tubercular drugs. | ||||
| Keywords | ||||
| Solanum nigrum; Hepatotoxicity; Isoniazid; Rifampicin; adiponectin; Rats | ||||
| References | ||||
|
ALSHAWSH, M.A., ABDULLA, M.A., ISMAIL, S. AND AMIN, Z.A. 2011. Hepatoprotective Effects of Orthosiphon stamineus Extract on Thioacetamide-Induced Liver Cirrhosis in Rats. Evidence-based complementary and alternative medicine 2011: 103039.
ANIL KUMAR, S., HIMA KUMARI, P., SHRAVAN KUMAR, G., MOHANALATHA, C., KAVI KISHOR, P. B. 2015. Osmotin: a plant sentinel and a possible agonist of mammalian adiponectin. Frontiers in plant science 6:163. doi:10.3389/fpls.2015.00163.
ANTUNA-PUENTE B., FEVE B., FELLAHI S., BASTARD J.P. 2008. Adipokines: The missing link between insulin resistance and obesity. Diabetes Metab 34(1): 2-11.
ARULMOZHI, V., KRISHNAVENI, M., MIRUNALINI, S. 2012. Protective effect of Solanum nigrum fruit extract on the functional status of liver and kidney against ethanol induced toxicity. J Biochem Tech 3(4):339-343.
BAIS, B., AND SAIJU, P. 2014,. Ameliorative effect of Leucas cephalotes extract on isoniazid an rifampicin-induced hepatotoxicity. Asian Pacific Journal of Tropical Biomedicine 4(2):S633-S638.
BELFIELD, A. AND GOLDBERG, D. 1971. Colorimetric determination of alkaline phosphatase activity. Enzyme 12(5): 561-568.
BHAVANI, R., GEETHA, G., SANTHOSHKUMAR, J., RAJESHKUMAR, S. 2015. Evaluation of Antibacterial action and Hepatoprotective efficiency of Solanum nigrum leaves extract on acetaminophen induced hepatotoxicity. Research J. Pharm. and Tech. 8(7): 893-900.
CARLETON, H.M., DRURY, R.A.B., WALLINGTON, E.A. 1980. Carleton's histological technique. Journal of clinical pathology 4th edn. Pp. 116.
CHALASANI, N., FONTANA, R.J., BONKOVSKY, H.L., WATKINS, P.B., DAVERN, T. SERRANO, J., YANG, H., ROCHON, J. 2008. Drug Induced Liver Injury Network (DILIN), Causes, clinical features, and outcomes from a prospective study of drug-induced liver injury in the United States. Gastroenterology 135:1924–1934.
CHO, J.Y., KIM, P.S., PARK, J., YOO, E.S., BAIK, K.U., KIM, Y.K. AND PARK, M.H. 2000. Inhibitor of tumor necrosis factor-alpha production in lipopolysaccharide-stimulated RAW264.7 cells from Amorpha fruticosa. J Ethnopharmacol., 70:127–133.
CHOI, J.H., CHOI, C.Y., LEE, K.J., HWANG, Y.P., CHUNG, Y.C., JEONG, H.G. 2009. Hepatoprotective effects of an anthocyanin fraction from purple-fleshed sweet potato against acetaminopheninduced liver damage in mice. J. Med. Food 12: 320–326.
Choi, J.H., Kim, D.W., Yun, N., Choi, J.S., Islam, M.N., KIM, Y.S., LEE, S.M. 2011. Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice. J. Nat. Prod. 74: 1055–1060.
DANIEL, T.M. 2006. Wilhelm Conrad Rötgen and the advent of thoracic radiology. Int. J. Tuberc. Lung. Dis., 10(11):1212-4.
DE SILVA, H.A., SAPARAMADU, P.A., THABREW, M.I., PATHMESWARAN, A., FONSEKA, M.M., DE SILVA, H.J. 2003. Liv-52 in alcoholic liver disease: a prospective, controlled trial. J. Ethnopharmacol., 84: 47–50.
DEHMLOW, C., ERHARD, J., DE GROOT, H. 1996. Inhibition of Kupffer cell functions as an explanation for the hepatoprotective properties of silibinin. Hepatology. 23:749–754.
DING, X., ZHU, F., YANG, Y., LI, M. 2013. Purification, antitumor activity in vitro of steroidal glycoalkaloids from black nightshade (Solanum nigrum L.) Food Chemistry. 141(2): 1181–1186.
DUFOUR, D.R., LOTT, J.A., NOLTE, F.S., GRETCH, D.R., KOFF, R.S., ET AL. 2000. Diagnosis and monitoring of hepatic injury: II. Recommendations for use of laboratory tests in screening, diagnosis and monitoring. Clin Chem 46: 2050-2068.
ELSHARKAWY, A.M. AND MANN, D.A. 2007. Nuclear factor-kappaB and the hepatic inflammation-fibrosis-cancer axis. Hepatology 46(2): 590-597.
Encyclopedia.com. 17 Sep. 2019. "milk thistle." A Dictionary of Food and Nutrition. . https://www.encyclopedia.com.
FALLAH HUSEIN, H.S.M, ALAVIAN HESHMAT, R., HEYDARI, M.R., ABOLMAALI, K. 2005. The efficacy of Liv-52 on liver cirrhotic patients: A randomized, double-blind, placebo-controlled first approach Phytomedicine 12(9): 619-624.
FRÜHBECK, G., CATALÁN, V., RODRÍGUEZ, A., RAMÍREZ, B., BECERRIL, S., SALVADOR, J.,PORTINCASA, P., COLINA, I., GÓMEZ-AMBROSI, J. 2017. Involvement of the leptin-adiponectin axis in inflammation and oxidative stress in the metabolic syndrome. Scientific Reports.7, pp. 6619.
GEAGEA, A.G., MALLAT, S., MATAR, C.F., ZERBE, R., FILFILI, E., FRANCIS, M., HAIDAR, H., JURJUS, A. 2018. Adiponectin and Inflammation in Health and Disease: An Update. 5:20-32.
GEORGIEVA, N., GADJEVA, V., TOLEKOVA, A. 2004. New isonicotinoylhydrazones with SSA protect against oxidative-hepatic injury of isoniazid. TJS 2:37–43
GIBONEY, P.T. 2005. "Mildly Elevated Liver Transaminase Levels in the Asymptomatic Patient". Am Fam Physician. 71 (6): 1105–10. PMID 15791889.
GUPTA, A. K., GANGULY, P., MAJUMDER, U. K., GHOSAL, S. 2009. hepatoprotective and antioxidant effects of total extracts and stereoidal saponins of Solanum xanthocarpum and Solanum nigrum in paracetamol induced hepatotoxicity In rats. Pharmacologyonline 1: 757-768.
HASSAN, H.A. AND EL-GENDY, A.M. 2003. Evaluation of silymarin and / or ginger effect on induced hepatotoxicity by carbon tetrachloride in male albino rats. The Egyptian Journal of Hospital Medicine 12: 101 – 112. I.S.S.N: 12084 1687-2002.
HAYDEN, F.G. 2006. Antiviral Agents ( Nonretroviral ): In: Goodman and Gilmans The Pharmacological Basics of Therapeutics, Goodmans L S, Gilman A, Brunton L L, Lazo J S And Parker K L. (Eds.) 11th Edn., McGraw-Hill Publication Co. Inc., New York, USA., ISBN-13: 9780071422802, pp: 1243-1271.
HEO, K.S., AND LIM, K.T., 2004. Antioxidative effects of glycoprotein isolated from Solanum nigrum L. J Med Food 7(3):349-57.
HSIEH, C.C., FANG, H.L., LINA, W.C. 2008. Inhibitory effect of Solanum nigrum on thioacetamide-induced liver fibrosis in mice. J. Ethnopharmacol. 119: 117–121.
HUSSEIN, O.E., GERMOUSH, M.O., MAHMOUD, A.M. 2016. Ruta graveolens Protects Against Isoniazid/Rifampicin-Induced Nephrotoxicity through Modulation of Oxidative Stress and Inflammation. Glob J Biotechnol Biomater Sci 1(1): 017-022.
ILYAS, N., SADIQ, M., JEHANGIR, A. 2011. Hepatoprotective effect of garlic (allium sativum) and milk thistle (silymarin) in isoniazid induced hepatotoxicity in rats. Biomedica. 27: 166 – 170.
JADHAV, V.B., THAKARE, V.N., SURALKAR, A.A., DESHPANDE, A.D., NAIK, S.R. 2010. Hepatoprotective activity of Luffa acutangula against CCl4 and rifampicin induced liver toxicity in rats: A biochemical and histopathological evaluation. Indian J Exp Biol.48:822–9. [PubMed] [Google Scholar]
JAIN, R., SHARMA, A., GUPTA, S., SARETHY, I.P., GABRANI, R., 2011. Solanum nigrum: Current Perspectives on Therapeutic Properties Alternative Medicine Review Monograph 16(1):78-85.
JAINU, M. AND DEVI, C.S. 2006. Antiulcerogenic and ulcer healing effects of Solanum nigrum (L.) on experimental ulcer models: possible mechanism for the inhibition of acid formation. J Ethnopharmacol. 104(1-2):156-63.
JEYAKUMAR, R., RAJESH, R., MEENA, B., RAJAPRABHU, D., GANESAN, B., BUDDHAN, S., ANANDAN, R. 2008. Antihepatotoxic effect of Picrorhiza kurroa on mitochondrial defense system in antitubercular drugs (isoniazid and rifampicin)-induced hepatitis in rats. Journal of Medicinal Plants Research 2(1): 017-019.
JIAN, L., QINGWANG, L., TAO, F., TAO, Z., KUN, L., RUI, Z., ZENGSHENG, H., DAWEI, G. 2007. Antitumor activity of crude polysaccharides isolated from Solanum nigrum Linne on U14 cervical carcinoma bearing mice. Phytother. Res. 21(9): 832-840.
KHALILI, H., DASHTI-KHAVIDAKI, S., RASOOLINEJAD, M., REZAIE, L., ETMINANA, M. 2009. Anti-tuberculosis drugs related hepatotoxicity: Incidence, risk factors, pattern of changes in liver enzymes and outcome. DARU 17: 163-167.
KISHORE, P.V., PALAIAN, S., PAUDEL, R., MISHRA, P., PRABHU, M., SHANKAR, P.R. 2007. Drug induced hepatitis with anti-tubercular chemotherapy: Challenges and difficulties in treatment. Kathmandu University Medical Journal 5, 2 (18):256-260.
KOJIMA, S., FUNAHASHI, T., SAKAMOTO, T., MIYAMOTO, S., SOEJIMA, H., HOKAMAKI, J., ET AL, 2003. The variation of plasma concentrations of a novel, adipocyte derived protein, adiponectin, in patients with acute myocardial infarction. Heart 89(6): 667.
KOKATE, C.K., PUROHIT, A.P., GOKHALE, S.B. 2002. Textbook of pharmacognosy, Nirali prakasan: Pune. 18:1-4 pp.
KUMAR, A., 2012. A review On Hepatoprotective Herbal Drugs. Int. J. Res.Pharm.chem., 2(1) 92-102.
LEE, S-J., KO, J-H., LIM, K. AND LIM, K-T. 2005. 150 kDa glycoprotein isolated from Solanum nigrum Linne enhances activities of detoxicant enzymes and lowers plasmic cholesterol in mouse. Pharmacological Research51(5): 399–408.
LIAN, Y., ZHAO, J., XU, P., WANG, Y., ZHAO, J. ET AL. 2013. Protective Effects of Metallothionein on Isoniazid and Rifampicin-Induced Hepatotoxicity in Mice. PLoS ONE 8(8): e72058. doi:10.1371/journal.pone.0072058.
LIN, H.M., TSENG, H.C., WANG, C.J., LIN, J.J., LO, C.W., CHOU, F.P. 2008. Hepatoprotective effects of Solanum nigrum Linn extract against CCl4-induced oxidative damage in rats. Chem. Biol. Interact. 171(3) 283-293.
LIN, H.Z., YANG, S.Q., ZELDIN, G., DIEHL, A.M. 1998. Chronic ethanol consumption induces the production of tumor necrosis factor-alpha and related cytokines in liver and adipose tissue. Alcohol. Clin. Exp. Res. 22(Suppl.):231S–237S.
LIU, J., ZHANG, H., ZHANG, Y., CHAI, T., 2013. Silicon attenuates cadmium toxicity in Solanum nigrum L. by reducing cadmium uptake and oxidative stress.Plant Physiology and Biochemistry (68)1–7.
LV P., LUO H-S., ZHOU X-P., XIAO, Y-J., PAUL, S.C., SI, X-M., ET AL. 2007.Reversal Effect of Thalidomide on Established Hepatic Cirrhosis in Rats via Inhibition of Nuclear Factor-κB/Inhibitor of Nuclear Factor-κB Pathway. Archives of medical research 38(1): 15-27.
MAKHLOUF, H.A., HELMY, A., FAWZY, E., EL-ATTAR, M., RASHED, H.A.G. 2008. A prospective study of antituberculous drug-induced hepatotoxicity in an area endemic for liver diseases. Hepatol Int 2:353–360. DOI 10.1007/s12072-008-9085-y
MASAKI, T., CHIBA, S., TATSUKAWA, H., YASUDA, T., NOGUCHI, H., SEIKE, M. YOSHIMATSU, H. 2004. Adiponectin Protects LPS-Induced Liver Injury Through Modulation of TNF- α in KK-Ay Obese Mice. Hepatology 40:177–184.
MBAGWU, F.N., NWACHUKWU, C.U., OKORO, O.O. 2007. Root Anatomical Studies on Solanum Macrocarpum and Solanum nigrum (Solanaceae): Journal of American Science, 3(3): :1-4. (ISSN: 1545-1003). DOI:10.4314/ajb.v8i17.62144
MOSCHEN, A.R., WIESER, V., TILG, H. 2012. Adiponectin: Key Player in the Adipose Tissue- Liver Crosstalk. Current Medicinal Chemistry 19(32), 5467-5473.
MUJAHID, M., HUSSAIN, T., SIDDIQUI, H.H., HUSSAIN, A. 2017. Evaluation of hepatoprotective potential of Erythrina indica leaves against antitubercular drugs induced hepatotoxicity in experimental rats. Journal of Ayurveda and Integrative Medicine 8:7-12.
NANASHIMA, K., MAWATARI, T., TAHARA, N., HIGUCHI, N., NAKAURA, A. ET AL, 2012. Genetic variants in antioxidant pathway: risk factors for hepatotoxicity in tuberculosis patients. Tuberculosis 92: 253-259.
OHKAWA, H., OHISHI, N., YAGI, K., 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry 95(2): 351-358.
OZER, J., RATNER, M., SHAW, M., BAILEY, W., SCHOMAKER, S., ET AL. 2008. The current state of serum biomarkers of hepatotoxicity. Toxicology 245: 194-205.
PAGLIA, D.E. AND VALENTINE, W.N. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70: 158 – 169.
PAL, R., VAIPHEI, K., SIKANDER, A., SINGH, K., RANA, S.V. 2006. Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats World J Gastroenterol 12(4): 636-639.
PENNICA, D., NEDWIN, G.E., HAYFLICK, J.S., SEEBURG, P.H., DERYNCK, R., PALLADINO, M.A. ET AL. 1984. Human tumour necrosis factor: Precursor structure, expression and homology to lymphotoxin. Nature 312(5996), 724–729.
PÉREZ-JIMÉNEZ, J., SAURA-CALIXTO, F. 2008. Grape products and cardiovascular disease risk factors. Nutr. Res. Rev. 21: 158–173.
PRABAKAN, M., ANANDAN, R., DEVAKI, T., 2000. Protective effect of Hemidesmusindicus against rifampicin and isoniazid-induced hepatotoxicity in rats. Fitoterapia 71: 55-59.
QADER, G.I., AZIZ, R.S., AHMED, Z.A., ABDULLAH, Z.F., HUSSAIN, S.A. 2014. Protective Effects of Quercetin against Isoniazid and Rifampicin Induced Hepatotoxicity in Rats. American Journal of Pharmacological Sciences 2(3) 56-60.
RAJU, K., ANBUGANAPATHI, G.,GOKULAK RISHNAN , V.,RAJKAPOOR, B., JAYAKAR, B., MANIAN, S. 2003. Effect of Dried Fruits of Solanum nigrum LINN against CCl4-Induced Hepatic Damage in Rats. Biol. Pharm. Bull. 26(11): 1618-1619.
REITMAN, S. AND FRANKEL, S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28(1) 56-63.
RIBEIRO, P.S., CORTEZ-PINTO, H., SOLÁ, S., CASTRO, R.E., RAMALHO, R.M., BAPTISTA, A. ET AL. 2004. Hepatocyte apoptosis, expression of death receptors, and activation of NF-κB in the liver of nonalcoholic and alcoholic steatohepatitis patients. The American journal of gastroenterology 99(9): 1708-1717.
SALAMA, A.A.A., FAYED, A.M., ATTIA, T.A., ELBATRAN, S.A., ISMAIEL, E.I., HASSAN, A. 2018. Protective Effects of Moringa oleifera extract on Isoniazid and Rifampicin Induced Hepatotoxicity in Rats: Involvement of Adiponectin and Tumor Necrosis Factor-α. Egypt. J. Vet. Sci. 49 (1): 25 – 34.
SANKARAN, M. 2012. Protective effect of Solanum nigrum fruit extract on the functional status of liver and kidney against ethanol induced toxicity. Journal of Biochemical Technology 3(4): 339-343.
SANTHOSH, S., SINI, T.K., ANANDAN, R., MATHEW, P.T. 2007. Hepatoprotective activity of chitosan against isoniazid and rifampicin-induced toxicity in experimental rats. Eur. J. Pharmacol. 572:69-73.
SHANKER, G., SYVERSEN, T., ASCHNER, J.L., ASCHNER, M. 2005. Modulatory effect of glutathione status and antioxidants on methylmercury-induced free radical formation in primary cultures of cerebral astrocytes. Brain Res. Mol. Brain Res. 137, 11-22.
SHIH, T.Y., HO, S.C., HSIONG, C.H., HUANG, T.Y., HU, O.Y. 2013. Selected pharmaceutical excipient prevent isoniazid and rifampicin induced hepatotoxicity. Curr Drug Metab. 14(6):720-8.
SHIVAKUMAR, S.P. AND VIDYASAGAR, G.M. 2013. Ethnomedicinal plants used in the treatment of skin diseases in Hyderabad Karnataka region, Karnataka, India, Asian Pacific Journal of Tropical Biomedicine 3(11): 882-886.
SON, Y.O., KIM, J., LIM, J.C., CHUNG, Y., CHUNG, G.H., LEE, J.C. 2003. Ripe fruits of Solanum nigrum L. inhibits cell growth and induces apoptosis in MCF-7 cells Food and Chemical Toxicology 41(10):1421-28.
SRIDHAR, T.M., JOSTHNA, P. AND NAIDU, C.V. 2011. Antifungal Activity, Phytochemical Analysis of Solanum nigrum (L.) - An Important Antiulcer Medicinal Plant .Journal of Ecobiotechnology, 3(7): 11-15.
STEELE MA, BURK RF, DESPREZ RM .1991. Toxic hepatitis with isoniazid and rifampin. A meta-analysis. Chest 99: 465-471.
SUBASH, K.R., RAMESH, K.S., CHARIAN, B.V., BRITTO, F., RAO, N.J., VIJAYKUMAR, S., 2011. Study of hepatoprotective activity of Solanum nigrum and Cichorium intybus. International Journal of Pharmacology 7 (4), 504-509.
SWAROOP, T.V.S.S. AND SHIVALINGE, G.K.P., 2012. Hepatotoxicity Mechanisms and its Biomarkers. International Journal of Pharmaceutical And Chemical Sciences 1 (2), 675-682.
TANG, M., POTTER, J., MEZEY, E., 2002. leptinenhance the effect of transforming growth factorbeta in increasing type I collagen formation. Biochem Biophys Res Commun 297, 906-911.
TAYAL, V., KALRA, B.S., AGARWAL, S., KHURANA, N., GUPTA, U., 2007. Hepatoprotective effect of tocopherol against isoniazide and rifampicin induced hepatotoxicity in albino rat. Indian Journal of Experimental Biology 45:1031-1036.
WALTERS, M.I. AND GERARDE, H., 1970. An ultramicromethod for the determination of conjugated and total bilirubin in serum or plasma. Microchemical Journal 15(2): 231-243.
WANG, L., HUANG, Q. H., LI, Y. X., HUANG, Y. F., XIE, J. H., XU, L. Q., SU ZR, ZENG HF, CHEN, J. N. 2018. Protective effects of silymarin on triptolide-induced acute hepatotoxicity in rats. Molecular medicine reports, 17(1), 789–800. doi:10.3892/mmr.2017.7958.
XU, A., WANG, Y., KESHAW, H., XU, L.Y., LAM, K.S.L., COOPER, G.J.S. 2003. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice. J. Clin. Invest.112:91–100.
YAMAUCHI, T., NIO, Y., MAKI, T., KOBAYASHI, M., TAKAZAWA, T., IWABU, M., OKADA-IWABU, M., KAWAMOTO, S., KUBOTA, N., KUBOTA, T., ITO, Y., KAMON, J., TSUCHIDA, A., KUMAGAI, K., KOZONO, H., HADA, Y., OGATA, H., TOKUYAMA, K., TSUNODA, M., IDE, T., MURAKAMI, K., AWAZAWA, M., TAKAMOTO, I., FROGUEL, P., HARA, K., TOBE, K., NAGAI, R., UEKI, K., KADOWAKI, T. 2007. Targeted disruption of AdipoR1 and AdipoR2 causes abrogation of adiponectin binding and metabolic actions. Nat Med. 13(3):332-9.
YOKOTA, T., ORITANI, K., TAKAHASHI, I., ISHIKAWA, J., MATSUYAMA, A., OUCHI, N. ET AL. 2000. adiponectin, a novel member of soluble defense collagens, negatively regulates the growth of myelo-monocytic progenitors and the functions of macrophages. Blood 96:1723-1732.
YOUSEF, M.I., OMAR, S.A., EL-GUENDI, M.I., ABDELMEGID, L.A. 2010. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food Chem. Toxicol. 48, 3246–3261.
YU, C.H., XU, C.F., YE, H., LI, L., LI, Y.M. 2010. Early mortality of alcoholic hepatitis: A review of data from placebo controlled clinical trials. World J. Gastroentrol. 16: 2435-2439.
ZAKARIA, Z.A., GOPALAN, H.K., ZAINAL, H. ET AL. 2006. Antinociceptive, anti-inflammatory and antipyretic effects of Solanum nigrum chloroform extract in animal models. Yakugaku Zasshi 126:1171-1178.
ZHANG, W., HONG, R., TIAN, T., 2013. Silymarin's Protective Effects and Possible Mechanisms on Alcoholic Fatty Liver for Rats. Biomolecules & therapeutics 21(4): 264–269. doi:10.4062/biomolther.2013.020.
ZUBAIR, M., RIZWAN, K., RASOOL, N., AFSHAN, N., SHAHID, M., AHMED V. 2011. Antimicrobial potential of various extract and fractions of leaves of Solanum nigrum International Journal of Phytomedicine, 3: 63-67. | ||||
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