Evaluation Of Bivalent Inactivated Infectious Bronchitis Viral Vaccine Prepared From Local Isolates | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 2, Volume 4, Issue 2, October 2019, Page 6-17 PDF (863.43 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2019.63446 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Reda R. Fathy   1; Magda M.A. Moustafa2; Zyan K.A2; El boraay I.M1; Susan S. El-Mahdy3
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| 1Department of Avian and rabbit diseases, Faculty of Veterinary Medicine, Benha University, Egypt. | ||||
| 2Department of Avian and rabbit diseases, Faculty of Veterinary Medicine, Benha University, Egypt | ||||
| 3Central Lab. for Evaluation of Vet. Biologics, Absassia, Cairo, Egypt | ||||
| Abstract | ||||
| Infectious Bronchitis (IB) is currently one of the most important diseases in poultry flocks all over the world causes huge economic losses in poultry industry. Many IBV outbreaks associated with respiratory distress, nephropathy, and high mortalities were attributed to the circulation of new nephropathogenic IBV variant 2 strains. This study was conducted to development of bivalent inactivated IBV vaccine by using the local classical and variant isolates (KP279995/2014 and KP279998/2014 respectively) which isolated from poultry farms in Egypt as previous surveillance study during IBV outbreak from Al-Sharkia and Al-Qalubia governorates. The locally prepared vaccine formulated by using formalin for inactivation and MontanideTM ISA71 RVG as oil adjuvant. The prepared bivalent inactivated (IBV) vaccine was tested for sterility, safety and potency, the efficacy of the prepared vaccine was applied in specific pathogen free chicks (SPF) for monitoring the antibody titers by using of Enzyme Linked Immuno Sorbent Assay (ELISA) post vaccination with estimation of shedding parameters by Real-Time RT-PCR in challenging birds with 104.5EID50/dose challenge IBV strains (classical and/or variant). The results revealed that the prepared vaccine free from any bacterial or mycotic contamination also safe after double dose inoculation in SPF chicks. The efficacy of inactivated vaccine showed from 92% to 96% protection against homologous challenge based on assessment parameters. This confirms that poultry industry can be protected from IB disease if using locally isolates in preparing of inactivated vaccine which reduce the economic losses caused by IB infection viruses in Egypt. | ||||
| Keywords | ||||
| Infectious bronchitis virus; Real-Time RT-PCR; inactivated vaccine | ||||
| References | ||||
|
ABDEL-MONEIM, A.S., El-KADY, M.F., LADMAN, B.S., GELB, J.J 2006 . S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt. Virol. J., 3: 78-
ABDEL-MONEIM, A.S., AFIFI, M.A.,El-KADY, M.F. 2012. Emergence of a novel genotype of avian infectious bronchitis virus in Egypt. Arch. Virol1.57, 2453–2457.
ABOZEID, H. H., PAIDURAI, A. , KHATTAR, S. K. , AFIFI, M. A. , El-KADY, M. F. , El-DEEB, A. H. , SAMAL, S. K. 2017. Complete genome sequences of two avian infectious bronchitis viruses isolated in Egypt: evidence for genetic drift and genetic recombination in the circulating viruses. Infect. Genet. Evol., 53,7–14.
AHMED, A., WALID, H. K, MOHAMED, A. Z. , MAGDY, E. , EL-KADY,M.F. 2018. Safety and efficacy of attenuated classic and variant 2 infectious bronchitis virus candidate vaccines. Poultry Science 0:1–7
BEARD,C.W. 1989.Isolation and identification of avian pathogens. Editorial committee of American association of avian pathologists 3rd edition ,Kendall hunt publishing Co.,Dubuque.IA,110-113.
BEN AROUS,J. D.,NORKINAC,S.,BORISON, S., ROZDESTVENSIY,V., ROZHDESTVENSKIY, T., GAUCHERON, I. , DUPUIS, L. J. 2013. MontanoidTM ISA71 RVG:A robust and flexible adjuvant formulation for potent and stable poultry vaccines. A Review. Egg-Broiler-INDIA, S Poultry Business Magazine. 6-9
CALLISON, S.A., D.A. HILT, T.O. BOYNTON, .F. SAMPLE, R. ROBISON, D.E. SWAYNE, AND M.W. JACKWOOD. (2006): Development and evaluation of a real-time Taqman RT-PCR assay for the detection ofinfectious bronchitis virus from infected chickens. J Virol Methods. 138:60–65.
CAVANAGH, D. 2007. Coronavirus avian infectious bronchitis virus .Vet. Res. 38, 279-281.
CLARKE,J.K., MCFERRAN,J.B. , GAY,F.W. 1972. Use of allantoic cells for the detection of avian infectious bronchitis virus. Arch.Gesamate virusforsch., 36,62-70.
CODE OF AMERICAN FEDERAL REGULATION. (CFR) . 2017. published by office of the federal register national archives records service, Animal and animal products.
COOK, J.K., JACK WOOD, M.W. , JONES, R. C. 2012. The long view: 40 years of infectious bronchitis research. Avian Pathology, 41, 3, 239-250.
DEGEN ,W.G., JANSEN T, SCHIJNS, V.E. 2003. Vaccine adjuvant technology: from mechanistic concepts to practical applications. Expert Rev Vaccines; 2.2, 327-335.
EGYPTIAN STANDARD REGULATIONS FOR EVALUATION OF VETERINARY BIOLOGICS .2017. The Egyptian standard regulations for the central laboratory of veterinary Biologics, 1, 2nd Ed.
EL-SAYED,M.H. 2014 .Improving the physical characters and immunological response of locally prepared oil emulsion ND vaccine, PH.D. Thesis, Veterinary virology, Fac. Vet.Med., Benha university, Egypt
EUROPEAN PHARMACOPOEIA. 2013. 2.6.1(Bacterial and fungal sterility) 2.6.5.8th edition. Test for extraneous virus using cell culture 2.6.7 Mycoplasma, culture method.
EUROPEAN PHARMACOPOEIA .2017.Avian infectious bronchitis vaccine (inactivated). 9th edition European Directorate for the Quality of Medicines and HealthCare (EDQM), Council of Europe, Strasbourg, France, 1007–1008
GELB, J., ROSENBERGER, J.K., FRIES, P.A., CLOUD, S.S., ODOR, E.M., DOHMS, J.E., JAEGER, J.S. 1991. Protection afforded infectious bronchitis virus-vaccinated sentinel chickens raised in a commercial environment. Avian Diseases, 33, 764-769.
HASSAN, K.E., SHANY, S.A., ALI, A., DAHSHAN, A.H., El-SAWAH, A.A. , El-KADY, M.F. 2016. Prevalence of avian respiratory viruses in broiler flocks in Egypt. Poult. Sci., 95, 1271–1280.
HUO, Y.F., HUANG, Q.H., Lu, M., Wu, J.Q., Lin, S.Q., Zhu, F., Zhang, X.M., Huang, Y.Y., Yang, S.H. , Xu, C.T. 2016. Attenuation mechanism of virulent infectious bronchitis virus strain with QX genotype by continuous passage in chicken embryos. Vaccine, 34, 83–89.
JACKWOOD, M.W. 2012. Review of infectious bronchitis virus around the world. Avian Dis. 56 ,4:634–641.
JACKWOOD, M.W. and De Wit, J.J. 2013. Infectious Bronchitis: In Diseases of Poultry, 13thedition .Ames, IA Blackwell Publishing Professional. pp. 117–135.
JANSEN, T., HOFMANS, M.P, THEELEN, M.J. , MANDERS, F., Schijns,V.E. 2006. Structure- and oil type-based efficacy of emulsion adjuvants. Vaccine. 24:5400–5405.
KIM, B. Y., LEE, D. H. , JANG, J. H. , LIM, T. H. , CHOI, S. W. , YOUN, H. N. Park, J. K. , LEE, J. B. , PARK, S. Y. , CHOI, I. S. , SONG. C. S. 2013. Cross-protective immune responses elicited by a Korean variant of infectious bronchitis virus. Avian Dis. 57,667–670.
LADMAN, B.S., POPE C.R., ZIEGLER, A.F., SWIECZKOWSKI, T.,CALLAHAN, C.J. , DAVISON, S. , GELB, Jr. J. 2002.Protection of chickens after live and inactivated virus vaccination against challenge with nephropathogenic infectious bronchitis virus PA/ Wolgemuth/98. Avian Dis. 46.938–944.
LEE, H.J.,YOUN, H.N., KWON, J.S., LEE, Y.J.,Kim, J.H., LEE, J.B., Park, S.Y.,CHOI, I.S., SONG, C.S. 2010.Characterization of a novel live attenuated infectious bronchitis virus vaccine candidate derived from a Korean nephropathogenic strain. Vaccine, 28, 2887–2894.
MEIR,R.,MAHARAT,O.,FArRNUSHI,Y.,SIMANOV,L.2010. Development of a real-time Taqman® RT-PCR assay for the detection of infectious bronchitis virus in chickens and comparison of RT-PCR and virus isolation . Journal of Virological Methods, 163,190-194.
MOCKETT, A.P. and DARBYSHIRE, J.H. 1981.Comparative Studies with an enzyme –linked immunosorbent assay (ELISA) for antibodies to avian infectious bronchitis virus. Avian Pathol, 10, 1-10 .
MOUSTAFA, H.A. 2018. Trail for preparation of combined oil emulsion inactivated H5N1, H9N2 and IB vaccine. PH.D. Thesis, Veterinary virology, Fac. Vet.Med., Benha university, Egypt
OIE, 2018. Manual of Diagnostic Tests and Vaccines for Terrestrial Animal, Office International des Epizooties, Paris, France: , 443-455.chapter 2.3.2.World Organization for Animal Health.
REDA, R. F., El BORAAY, I.M., EL- SHORBAGY, M.A., Susan, S. 2015. A survey on presence of new strains of infectious bronchitis virus in some chicken farms of Egyptian Delta provinces during 2014. Benha Veterinary Medical Journal, 28, 2:248‐262.
REED, L.J. and MUENCH, H. 1938. A simple method for estimating fifty eprcent endpoint. Am. J. Hyg., 27, 493-496.
ROH, H.J., JORDAN, B.J., HILT, D.A.,JACKWOOD,M.W. 2014.Detection of infectious bronchitis virus with the use of real-time quantitative reverse transcriptase-PCR and correlation with detection in embryonated eggs. Avian Dis., 58, 398-403.
SEPPIC ,2012 . Montanide for avian vaccines, seppic poultry seminar,Dubai,2012.Seppic limited In corporation company.
SUSAN, S., El-HADY, M., SOLIMAN, Y. 2010. Isolation and characterization of nephropathogenic strain of infectious bronchitis virus in egypt. J. Am. Sci., 6, 669–674.
SUSAN, S., Ya, S., MM, E.-H. 2011.Preparation and Evaluation of Master Seed for infectious bronchitis vaccine from local variant isolate. Nat. Sci. 9, 145–150.
TORO, H., VAN SANTEN, V. L. GHETAS, A. M. JOINER, K. S. 2015. Cross-protection by infectious bronchitis viruses under controlled experimental conditions. Avian Dis. 59, 532–536.
VAlASTRO, V., Holmes, E. C., Britton, P., Fusaro, A., Jackwood, M. W., Cattoli, G. , Monne, I. 2016. S1 gene-based phylogeny of infectious bronchitis virus: An attempt to harmonize virus classification. Infect. Genet. Evol. 39,349-364.
WANG, C.H. and HUANG, Y.C. 2000. Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus. Archives of Virology, 145, 291-300.
ZANATY, A., NAGUIB, M.M., El-HUSSEINY, M.H., MADY, W., HAGAG, N., ARAFA, A.S.2016. The sequence of the full spike S1 glycoprotein of infectious bronchitis virus circulating in Egypt reveals evidence of intra-genotypic recombination. Arch. Virol., 161, 3583–3587.
ZHAO, Y., CHENG, J.L., LIU, X.Y., ZHAO, J., HU, Y.X., ZHANG, G.Z. 2015. Safety and efficacy of an attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine. Vet. Microbiol., 180, 49–58. | ||||
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