THE EFFECT OF MINERAL TRIOXIDE AGGREGATE ALONE AND IN COMBINATION WITH BIPHASIC CALCIUM PHOSPHATE ON HEALING OF MANDIBULAR DEFECTS IN RABBIT
Galal, G., Abd El Rehim, S., Karam, S. (2019). THE EFFECT OF MINERAL TRIOXIDE AGGREGATE ALONE AND IN COMBINATION WITH BIPHASIC CALCIUM PHOSPHATE ON HEALING OF MANDIBULAR DEFECTS IN RABBIT. EKB Journal Management System, 44(3), 77-81. doi: 10.21608/adjalexu.2019.63562
Gailan M. Galal; Samia S. Abd El Rehim; Sahar S. Karam. "THE EFFECT OF MINERAL TRIOXIDE AGGREGATE ALONE AND IN COMBINATION WITH BIPHASIC CALCIUM PHOSPHATE ON HEALING OF MANDIBULAR DEFECTS IN RABBIT". EKB Journal Management System, 44, 3, 2019, 77-81. doi: 10.21608/adjalexu.2019.63562
Galal, G., Abd El Rehim, S., Karam, S. (2019). 'THE EFFECT OF MINERAL TRIOXIDE AGGREGATE ALONE AND IN COMBINATION WITH BIPHASIC CALCIUM PHOSPHATE ON HEALING OF MANDIBULAR DEFECTS IN RABBIT', EKB Journal Management System, 44(3), pp. 77-81. doi: 10.21608/adjalexu.2019.63562
Galal, G., Abd El Rehim, S., Karam, S. THE EFFECT OF MINERAL TRIOXIDE AGGREGATE ALONE AND IN COMBINATION WITH BIPHASIC CALCIUM PHOSPHATE ON HEALING OF MANDIBULAR DEFECTS IN RABBIT. EKB Journal Management System, 2019; 44(3): 77-81. doi: 10.21608/adjalexu.2019.63562

THE EFFECT OF MINERAL TRIOXIDE AGGREGATE ALONE AND IN COMBINATION WITH BIPHASIC CALCIUM PHOSPHATE ON HEALING OF MANDIBULAR DEFECTS IN RABBIT

Alexandria Dental Journal
Article 15, Volume 44, Issue 3, December 2019, Page 77-81  XML PDF (350.1 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2019.63562
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Authors
Gailan M. Galal email 1; Samia S. Abd El Rehim2; Sahar S. Karam3
1Demonstrator of Oral Biology, Faculty of Dentistry, Alexandria University
2Professor of Oral Biology, Faculty of Dentistry, Alexandria University
3Professor and Head of Oral Biology department, Faculty of Dentistry, Alexandria University.
Abstract
INTRODUCTION: Critical sized bone defects above critical size do not heal completely by themselves and thus represent major clinical challenge to reconstructive surgery. Numerous bone substitutes have already been used to promote bone regeneration. Incorporation of bone grafts such as biphasic calcium phosphate (BCP) as osteoconductive materials has been proven to be effective in promoting bone healing in oral surgery. As it is considered a potential candidate for bone regenerating biomolecules which can help in rapid bone repair because of its composition, the biocompatible material "mineral trioxide aggregate"(MTA) is suggested to be promising. OBJECTIVES: To compare the efficacy of MTA on the process of bone healing alone and the combination of MTA with biphasic calcium phosphate MATERIALS AND METHODS: Sixteen rabbits will be divided into two experimental groups: (1) MTA, (2) MTA+BCP. Right and left critical size bone defects will be performed in the edentulous area of rabbit mandibles (diastema). Right side bone defects will be filled with MTA, while the left side bone defects will be filled with MTA in combination with BCP. The effect of both MTA and BCP on defect healing will be assessed histologically, histomorphometrically and immunohistochemically after 6 weeks postoperatively. RESULTS: Greater amount of new bone formation was noticed in the CSDs loaded with MTA and BCP. The amount of new bone formed was significantly higher in the MTA+BCP group when compared with the MTA group. Also bone cells activities appeared greater in the former group. CONCLUSIONS: MTA induces new bone formation via an osteoinductive action and can be added to bone grafts to enhance bone regenerative capacity
Keywords
Mineral trioxide aggregate; Biphasic Calcium phosphate; bone defects; Rabbit
References
  1. GOMES, C., et al. Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits. Arq. Bras. Med. Vet. Zootec. 2011, 63.4: 836-843.
  2. EZIRGANLI, Şeref, et al. The effect of local simvastatin application on critical size defects in the diabetic rats. Clin Oral Implants Res. 2014, 25.8: 969-976.
  3. Jang ES,Park JW,Kweon H,Lee KG,Kang SW,Baek DH,et al.Restortion of peri-implant defects in immediate implant installations by Choukroun platelet-rich fibrin and silk fibrin powder combination gaft. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109(6):831-6.
  4. Moore WR,Graves SE,Bain GI.Synthetic bone graft substitutes. ANZ J Surg. 2001;71(6):354-61.
  5. Giannoudis PV,Dinopoulos H,Tsiridis E.Bone substitutes: an update. Injury. 2005;36(3):S20-S27.
  6. JENSEN, Simon Storgård, et al. Evaluation of a novel biphasic calcium phosphate in standardized bone defects. A histologic and histomorphometric study in the mandibles of minipigs. Clin Oral Implants Res. 2007, 18.6: 752-760.
  7. KIM, S. H., et al. Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria. Int. J. Oral Maxillofac. Surg. 2015, 44.4: 535-542.
  8. Xu L,Lv K,Zhang W,Zhang X,Jiang X,Zhang F. The healing of critical-size calvarial bone defects in rat with rhPDGF-BB,BMSCs,and ß-TCP scffolds. J. Mater. Sci. Mater. Med. 2012;23(4):1073-84.
  9. Komabyashi T,Zhu Q.Innovative endodontic therapy for anti-inflammatory direct pulp capping of permanent teeth with a mature apex. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109;75-81.
  10. PINHEIRO, Antonio LB, et al. Does LED phototherapy influence the repair of bone defects grafted with MTA, bone morphogenetic proteins, and guided bone regeneration? A description of the repair process on rodents. Lasers Med. Sci. 2012, 27.5: 1013-1024.
  11. WOZNEY, John M.; ROSEN, Vicki. Bone morphogenetic protein and bone morphogenetic protein gene family in bone formation and repair. Clin. Orthop Relat. Res. 1998, 346: 26-37.
  12. CHEN, D., et al. Differential roles for bone morphogenetic protein (BMP) receptor type IB and IA in differentiation and specification of mesenchymal precursor cells to osteoblast and adipocyte lineages. J. Cell Biol. 1998, 142.1: 295-305.
  13. LIN, Shuxian, et al. The biological function of type I receptors of bone morphogenetic protein in bone. Bone Res. 2016, 4: 16005.
  14. ONISHI, T., et al. Distinct and overlapping patterns of localization of bone morphogenetic protein (BMP) family members and a BMP type II receptor during fracture healing in rats. Bone Res. 1998, 22.6: 605-612.
  15. Eleftheriadis E, Leventis MD, Tosios KI, Faratzis G, Titsinidis S, Eleftheriadi I, et al. Osteogenic activitiy of BETA-tricalcium phosphate in a hydroxyl sulphate matrix and demineralized bone matrix: a histological study in rabbit mandible. J Oral Sci 2010;52(3):377-84
  16. Yugoshi LI, Sala MA, Brentegani LG, Carvalho TLL. Histometric study of socket healing after tooth extraction in rats treated with diclofenac. Braz Dent J. 2002;13(2):92-6.
  17. Koh E, McDonald F, Pitt Ford T, Torabinejad M. Cellular response to mineral trioxide aggregate. J Endod. 1998;24: 543-547.
  18. Arens DE, Torabinejad M. Repair of furcal perforation with mineral trioxide aggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996;82: 84-88.
  19. Do Nascimento, Cássio, et al. Bone repair using mineral trioxide aggregate combined to a material carrier, associated or not with calcium hydroxide in bone defects. Micron. 2008;39.7: 868-874.
  20. Zhu, Qiang, et al. Adhesion of human osteoblasts on rootend filling materials. J Endod. 2000; 26.7: 404-406.
  21. PERINPANAYAGAM, Hiran; AL-RABEAH, Ebtehal. Osteoblasts interact with MTA surfaces and express Runx2. Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 2009;107.4: 590-596.
  22. Min K, Park H, Lee S, Park S, Hong C, Kim H, et al. Effect of Mineral Trioxide Aggregate on Dentin Bridge Formation and Expression of Dentin Sialoprotein and Heme Oxygenase-1 in Human Dental Pulp. J Endod. 2008: 106: 609-14.
  23. Al-Hezaimi K, Salameh Z, Al-Fouzan K, Al Rejaie M, Tay F. Histomorphometric and Micro-computed Tomography Analysis of Pulpal Response to three Different Pulp Capping Materials. J Endod. 2011; 37:507-12.
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