EFFECT OF OZONE ACTIVATED PLATELET RICH PLASMA ON THE MANAGEMENT OF GRADE II FURCATION INVOLVEMENT (COMPARATIVEEXPERIMENTALSTUDY) | ||||
| Alexandria Dental Journal | ||||
| Article 20, Volume 44, Issue 3, December 2019, Page 106-111 PDF (268.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjalexu.2019.63567 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Moustafa S. Abouyoussef  1; Ahmed M. Hommos2; Samia S. Omar3; Gehan S. Kotry2
| ||||
| 1Bachelor of Dental Science 2007, Faculty of dentistry, Misr International University, Egypt | ||||
| 2Professor of Periodontology, Faculty of Dentistry, Alexandria University, Egypt | ||||
| 3Professor of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt | ||||
| Abstract | ||||
| INTRODUCTION: Furcation defects are a frequent finding in periodontitis patients. Activated platelets release numerous proteins, among them adhesive glycoproteins and growth factors. Ozone (O3) is a triatomic molecule, consisting of three oxygen atoms, and its application in medicine and dentistry has been indicated for the treatment of different pathologies. AIM OF THE STUDY: The aim of this experimental study was to evaluate the effectiveness of combining Platelet Rich Plasma (PRP) and ozone therapy in the management of surgically created grade II furcation defects. MATERIALS AND METHODS: A split mouth design was carried out using six healthy mongrel dogs. A total of eighteen critical sized grade II furcation defects were surgically created in the third and fourth mandibular premolars, bilaterally. After reflecting a mucoperiosteal flap, the defects in the experimental side were treated with PRP activated by ozone, β-TCP and collagen membranes, whereas, the control side defects were treated with PRP, β-TCP and collagen membrane. The dogs were sacrificed after 4 and 8 weeks. Samples were dissected and prepared for histological evaluation. RESULTS: Histological results showed that PRP activated by ozone led to higher vascularization of the study samples at all time points; this was evident at four weeks in the form of numerous blood vessels entrapping RBCs and at eight weeks by the presence of red bone marrow and blood vessels entrapping RBCs within. Histomorphometry revealed that ozonated PRP accelerated bone regeneration at both time intervals, this acceleration was almost two –fold upon comparing test to controls. CONCLUSION: Activation of PRP by ozone is effective in enhancing angiogenesis and bone formation during periodontal regenerative therapy in grade II critical –sized furcation defectsin dogs | ||||
| Keywords | ||||
| Angiogenesis; β-TCP. Grade II furcation Periodontal regeneration; platelet rich plasma | ||||
| References | ||||
|
1. Raja S, Nath G, Emmadi P, Ramakrishnan, Ahathya. Treatment of an isolated furcation involved endodontically treated tooth - a case report. J Conserv Dent.2007; 10:129-33.
2. Albanese A, Licata ME, Polizzi B, Campisi G. Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration. Immun Ageing.2013; 10: 23. 3. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet Rich Plasma growth factors enhancement for bonegrafts. Oral Surg Oral Med Oral Pathol Oral RadiolEndod.1998; 85:638-46. 4. Kaigler D, Avila G, Wisner-Lynch L, Nevins ML, Nevins M, Rasperini G, et al. Platelet-Derived Growth Factor Applications in Periodontal and Peri-Implant Bone Regeneration. Expert OpinBiolTher.2011; 11:375-85. 5. Retzepi M, Donos N. Guided Bone Regeneration: biological principle and therapeutic applications. Clin Oral Implants Res.2010;21:567-76. 6. Iuliano L, Colavita AR, Leo R, Pratic`o D, Violi F. Oxygen free radicals and platelet activation. Free Rad Biol Med.1997; 22:999-1006. 7. Ebensberger U, Pohl Y, Filippi A. PCNA-expression of cementoblasts and fibroblasts on the root surface after extraoral rinsing for decontamination. Dent Traumatol.2002; 18:262-6. 8. Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod. 2004; 30:778- 81. 9. Ghaitth HM, Lotfy M, Soliman S, Fahmy RA. Quantitative Analysis of Interradicular Bone Regeneration after Use of Modified Perforated Collagen Membrane versus Occlusive Membrane in the Management of Grade II Furcation Defects in Dogs. Impact Factor.2015; 6:391. 10. Toffler M, Toscano N, Holtzclaw D, Dds D, Ehrenfest DD, Bullet N. Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery Milieu. JIACD. 2009; 1: 21-32.
11. Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral RadiolEndod.2009; 108:56e61.
12. Yilmaz D, Dogan N, Ozkan A, Sencimen M, Oral BE, Mutlu I. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing.A histological study in pigs. ActaCirúrgicaBrasileira.2014;29:59-68.
13. Suaid FF, Carvalho MD, Ambrosano GM, Nociti FH Jr, Casati MZ, Sallum EA. Platelet-rich plasma in the treatment of Class II furcation defects: a histometrical study in dogs. J Appl Oral Sci.2012;20:162-9.
14. Mansouri S, Ghasemi M, Darmian S, Pourseyediyan T. Treatment of Mandibular Molar Class II Furcation Defects in Humans With Bovine Porous Bone Mineral in Combination With Plasma Rich in Growth Factors. J Dent (Tehran). 2012;9:41-9.
15. Suaid FF, Carvalho MD, Ambrosano GMB, Junior FHN, Casati MZ, Sallum EA. Platelet-rich plasma in the treatment of Class II furcation defects: a histometrical study in dogs. J Appl Oral Sci. 2012; 20:162-9.
16. Giannobile WV, Hernandez RA, Finkelman RD, Ryan S, Kiritsy CP, D'Andrea M, et al. Comparative effects of platelet-derived growth factor-BB and insulin-like growth factor-I, individually and in combination, on periodontal regeneration in Macaca-fascicularis. J Periodontal Res.1996;31:301-12.
17. Sigurdsson TJ, Lee MB, Kubota K, Turek TJ, Wozney JM, Wikesjö UM. Periodontal repair in dogs: recombinant human bone morphogenetic protein-2 significantly enhances periodontal regeneration. J Periodontol.1995;66:131-8.
18. Polson AM, Garrett S, Stoller NH, Greenstein G, Polson AP, Harrold CQ, et al. Guided tissue regeneration in human furcation defects after using a biodegradable barrier: a multi-center feasibility study. J Periodontol.1995;66:377-85.
19. Jensen TB, Rahbek O, Overgaard S, Søballe K. No effect of platelet-rich plasma with frozen or processed bone allograft around noncemented implants. IntOrthop. 2005;29:67-72.
20. Ray AK, Jones AC, Carnes DL, Cochran DL, Mellonig JT, Oates TW., Jr Platelet- derived growth factor-BB stimulated cell migration mediated through p38 signal transduction pathway in periodontal cells. J Periodontol.2003;74:1320-28.
21. Bocci V, Valacchi G, Rossi R, Giustarini D, Paccagnini E, Pucci AM, et al. Studies on the biological effects of ozone: 9. Effects of ozone on human platelets. Platelets.1999;10:110-6.
22. Weglein A. Effect of autologous fibrin gel and platelet rich plasma activated by ozone versus those activated by calcium chloride on wound healing and prevention of infection in high risk cesarean sections: randomized controlled study. 2017. Available at: http://www.houstonsportsdoctor.com/blog/effect-of-autologous-fibrin-gel-platelet- rich-plasma-activated-by-ozone-versus-those-activated-by-calcium-chloride-on- wound-healing-prevention-of-infection-in-high-risk-cesarean-sections-randomized-controlled-study-10338.html. | ||||
|
Statistics Article View: 411 PDF Download: 488 |
||||
