Sublethal Effects of the Milky Latex of Sodom Apple, Calotropis procera (Alton) on the Growth, development, and some Physiological Aspects of the Greater Wax Moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae)
El-Hefny, A. (2019). Sublethal Effects of the Milky Latex of Sodom Apple, Calotropis procera (Alton) on the Growth, development, and some Physiological Aspects of the Greater Wax Moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). EKB Journal Management System, 10(10), 491-496. doi: 10.21608/jppp.2019.63674
A. A. El-Hefny. "Sublethal Effects of the Milky Latex of Sodom Apple, Calotropis procera (Alton) on the Growth, development, and some Physiological Aspects of the Greater Wax Moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae)". EKB Journal Management System, 10, 10, 2019, 491-496. doi: 10.21608/jppp.2019.63674
El-Hefny, A. (2019). 'Sublethal Effects of the Milky Latex of Sodom Apple, Calotropis procera (Alton) on the Growth, development, and some Physiological Aspects of the Greater Wax Moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae)', EKB Journal Management System, 10(10), pp. 491-496. doi: 10.21608/jppp.2019.63674
El-Hefny, A. Sublethal Effects of the Milky Latex of Sodom Apple, Calotropis procera (Alton) on the Growth, development, and some Physiological Aspects of the Greater Wax Moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). EKB Journal Management System, 2019; 10(10): 491-496. doi: 10.21608/jppp.2019.63674

Sublethal Effects of the Milky Latex of Sodom Apple, Calotropis procera (Alton) on the Growth, development, and some Physiological Aspects of the Greater Wax Moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae)

Journal of Plant Protection and Pathology
Article 4, Volume 10, Issue 10, October 2019, Page 491-496  XML PDF (473.36 K)
Document Type: Original Article
DOI: 10.21608/jppp.2019.63674
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Author
A. A. El-Hefny email
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Abstract
The lethal, sublethal and some physiological effects of crude latex of Sodom apple, Calotropis procera 9Alton) were assessed using the third instar larvae of the greater wax moth, Galleria mellonella (L.) as a model insect species. Latex showed LC50 of 37 μl latex/g diet after 48 hours of feeding on latex incorporated artificial diet. Larvae were continuously fed, until pupation, with artificial diet containing LC20 of latex. Feeding inhibition and accumulated larval mortality of 53 .0 ± 15% and 73 ±2.6%, respectively, were observed before pupation for the LC20 fed larvae. The incorporation of C. procera latex into the diet significantly prolonged the larval stage duration and decreased larval and pupal weight than untreated larvae.  Furthermore, latex significantly reduced relative consumption rate (RCR), relative growth rate (RGR) and efficiency of conversion of ingested food (ECI). While there were no significant effects of latex on activities of both invertase, and esterases (α and β Esterases) it showed a strong and significant inhibitory effect on α–amylase.  However, latex induced an obvious and significant increase in alkaline phosphatase activity. It is suggested that the antifeedant effect of C. procera latex could be due to the digestion inhibition through the inhibition of α–amylase activity, while a significant portion of the digested food were used by C. procera latex fed larvae for synthesis alkaline phosphatase. These results provide more evidences regarding the mode of action of the lethal and sublethal effects of C. procera latex on G. mellonella.as a model insect species.
Keywords
Calotropis procera; latex; Galleria mellonella; Growth; Development; Nuteation; physiology
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