Transcriptional activity of 3' deletion of the rat prolactin gene promoter
El-jaffry, S., Fehalbum, I., Aldawy, K., Hussein, M., A. A, H. (2013). Transcriptional activity of 3' deletion of the rat prolactin gene promoter. EKB Journal Management System, 9(1), 6-11. doi: 10.21608/blj.2013.63861
Salem El-jaffry; I. Fehalbum; Kh. Aldawy; Mohamed M.A. Hussein; Haytham. A. A. "Transcriptional activity of 3' deletion of the rat prolactin gene promoter". EKB Journal Management System, 9, 1, 2013, 6-11. doi: 10.21608/blj.2013.63861
El-jaffry, S., Fehalbum, I., Aldawy, K., Hussein, M., A. A, H. (2013). 'Transcriptional activity of 3' deletion of the rat prolactin gene promoter', EKB Journal Management System, 9(1), pp. 6-11. doi: 10.21608/blj.2013.63861
El-jaffry, S., Fehalbum, I., Aldawy, K., Hussein, M., A. A, H. Transcriptional activity of 3' deletion of the rat prolactin gene promoter. EKB Journal Management System, 2013; 9(1): 6-11. doi: 10.21608/blj.2013.63861

Transcriptional activity of 3' deletion of the rat prolactin gene promoter

Biochemistry Letters
Article 2, Volume 9, Issue 1, 2013, Page 6-11  XML PDF (910.72 K)
DOI: 10.21608/blj.2013.63861
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Authors
Salem El-jaffry1; I. Fehalbum1; Kh. Aldawy2; Mohamed M.A. Hussein2; Haytham. A. A2
1Department of Biochemistry, Faculty of Medicine, Tripoli, Libya
2Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt
Abstract
 




 Regulation of gene expression of prolactin gene still a point of study for many Scientifics so this work was designed to investigate the transcriptional activity of 3- deletion of the rat prolactin gene promoter. A plasmid containing the rPrl gene promoter (-423/+38) was restricted at the unique EcoR1 site of 3' deletion of the ePrl promoter sequences in rPrl (-423/+38). With suitable condition established prPrl (-423/+38) was EcoR1 restricted in preparative amounts and Bal31 treated for optimized time periods and the resulting DNA fragments pooled. The selected positives were then restricted with BamH1, Hind III and the presence of a Bam H1/ Hind III fragment 423 bp verified by agarose gel electrophoresis. The results shows that the most proximal high affinity Pit-1/GHF-1 binding element is not an absolute requirement for GC cell specific transcriptional activity and that the Pit-1/GHF-1 binding elements with sequence to -423, is sufficient to confer relatively high transcriptional drive, in orientation independent manner 
Keywords
prolactin; promoter; 3' deletion; Pit-1/GHF-1
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