Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea
Mar'ie, Z., Allam, M. (2019). Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea. EKB Journal Management System, 11(3), 109-116. doi: 10.21608/eajbsc.2019.63943
Zeinab Mar'ie; Mohammad Allam. "Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea". EKB Journal Management System, 11, 3, 2019, 109-116. doi: 10.21608/eajbsc.2019.63943
Mar'ie, Z., Allam, M. (2019). 'Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea', EKB Journal Management System, 11(3), pp. 109-116. doi: 10.21608/eajbsc.2019.63943
Mar'ie, Z., Allam, M. Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea. EKB Journal Management System, 2019; 11(3): 109-116. doi: 10.21608/eajbsc.2019.63943

Usage of Inter Simple Sequence Repeat (ISSR) Marker in Assessing the Genetic Variation of Six Parrotfish Species from the Egyptian Red Sea

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 10, Volume 11, Issue 3, December 2019, Page 109-116  XML PDF (1.05 MB)
Document Type: Original Article
DOI: 10.21608/eajbsc.2019.63943
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Authors
Zeinab Mar'ie1; Mohammad Allam2
11- Zoology Department, Faculty of Science, South Valley University, Egypt 2- Science Departments, Faculty of Education, Hurghada , South Valley University, Egypt
2Zoology Department, Faculty of Science, South Valley University, Egypt
Abstract
The report compared six parrotfish species (Scarus ferrugineus, Scarus psittacus, Scarus frenatus, Chlorurus sordidus, scarus genazotus and Cetoscarus bicolor) for molecular diversity and phylogenetic using eleven ISSR primers, the obtained results showed a total of 235 DNA fragments; 11 were common bands, 65 were specific bands and 159 were polymorphic bands. Additionally, the results indicated the largest similarity degree 58 between (Chlorurus sordidus and scarus genazotus), while the lowest similarity degree 35 was found between (Scarus frenatus and Chlorurus sordidus). The results that occurred from ISSR analysis found to be more reliable for differentiating species of fishes particularly parrotfish.   
Keywords
DNA; Genetic diversity; ISSR; Parrotfish; and PCR
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