Enhancement of Antibiotics activity by microbially synthesized silver nanoparticles | ||||
| African Journal of Biological Sciences | ||||
| Article 11, Volume 15, Issue 1, 2019, Page 137-153 PDF (613.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajbs.2019.64008 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mahmoud Abushiba 1; Gamal El-Sherbiny2; Saad Moghannem3; Mohamed Kalaba2; Einas El- Shatoury4; Ali Saeed5
| ||||
| 1Department of Biology, Faculty of Science, Azzaytuna University, Tarhuna, Libya | ||||
| 2Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt | ||||
| 3Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt. | ||||
| 4Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
| 5-Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Biological synthesis of nanoparticles is a promising approach that is comparatively inexpensive and eco-friendly. Among various microorganisms, actinobacteria have been found to be efficient in the synthesis of nanomaterials. In thisstudy, extracellular biosynthesis of silver nanoparticles (Ag-NPs) was investigated by mixing silver nitrate with culture filtrate of Streptomyces rochei strain MFA-37.The biosynthesized Ag-NPs were characterized by UV–Vis spectrophotometric analysis, and showed a peak of absorbance at 440 nm. Fourier transform infrared (FTIR) analysis showed amines and amides that are responsible for the stabilization of Ag-NPs. The morphology and size of nanoparticles were determined using high-resolution transmission electron microscopy, XRD and DLS techniques which indicated well dispersed, spherical Ag-NPs sized 2–20 nm, and the zeta potential of AgNPs reached -20.5 mV. Enhancement of antibacterial activity of fourteen antibiotics was conducted by combining antibiotics with synthesized AgNPs. The synergistic activities of AgNPs combination with antibiotics were determined using disc diffusion against two multi-drug resistant Staphylococcus aureus SA-185 and SA-325 recovered from Libya. The highest synergistic effects were 6.11 and 6.37 folds for Penicillin followed by 6.11 and 4.44 folds for Ciprofloxacin and 6.11 and 3.69 folds for Erythromycin for MRSA-185 and MRSA-325 , respectively. This activity of AgNPs and antibiotics could be valuable for the manufacture of hybrid drugs to kill or inhibit the MRSA infection especially in case of superficial injuries. | ||||
| Keywords | ||||
| MRSA; Ag-NPs; antibiotics; synergistic effects | ||||
|
Statistics Article View: 718 PDF Download: 363 |
||||
