SIMULTANEOUS DETERMINATION OF INTACT LOMEFLOXACIN AND CIPROFLOXACIN IN THE PRESENCE OF THEIR ACID DEGRADATION PRODUCTS | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 12, Volume 30, Issue 2, December 2007, Page 241-258 PDF (439.05 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2007.64205 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Sonia T. Hassib; Ramzia El-Bagary* ; Hanaa M. Hashem; Maha M. El-Hakim | ||||
| Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Egypt | ||||
| Abstract | ||||
| A reversed phase HPLC method was developed for the determination of lomefloxacin and its degradation product. In addition, two other methods have been developed for the determination of lomefloxacin hydrochloride (LF.HCl) and ciprofloxacin hydrochloride (CF.HCl) in presence of their acid induced degradation products. For the reversed phase HPLC method (determination of LF.HCl), the mobile phase used was a mixture of water: acetonitrile: triethylamine (80:20:0.6, v/v/v) adjusted to pH 3.0 with o-phosphoric acid. The flow rate was 1.5 ml/min. and the detection was carried out at 328 nm. The linearity range was found to be 0.5-6 μg / 20 μl for LF.HCl. The limits of detection and quantification (LOD & LOQ) were 0.22 μg / 20 μl & 0.74 μg / 20 μl respectively. The second method was densitometric method for the determination of both LF.HCl and CF.HCl, the developing system used was a mixture of methanol and ammonia buffer (80:20, v/v). Detection was carried out at 288 nm & 279 nm. for intact LF.HCl and CF.HCl respectively. The linearity ranges were found to be 1-6 μg / 10 μl & 0.25-2.5 μg / 10 μl for intact LF.HCl and CF.HCl respectively. LOD & LOQ were 0.1, 0.34 μg / 10 μl & 0.05, 0.18 μg / 10 μl for both drugs, respectively. The third method was derivative spectrophotometric method for the determination of (LF.HCl) & (CF.HCL). The linearity ranges were found to be 2-8 μg/ml & 5-12 μg/ml for LF.HCl and CF.HCl respectively. LOD & LOQ were 0.39 μg, 1.29 μg/ml & 1.03, 3.45 μg/ml for LF.HCl and CF.HCl, respectively. Separation and identification of the acid degradation products of lomefloxacin hydrochloride and ciprofloxacin hydrochloride were carried out. The three described methods proved to be sensitive, precise and applicable to both dosage forms and laboratory prepared mixtures of the intact drugs and their acid degradation products. | ||||
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