PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T
HAGAGGI, N., HEZAYEN, F., ABDULRAOUF, U. (2013). PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T. EKB Journal Management System, 24(Issue 2-C), 93-108. doi: 10.21608/absb.2013.6422
NOURA HAGAGGI; FRANCIS F. HEZAYEN; USAMA M. ABDULRAOUF. "PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T". EKB Journal Management System, 24, Issue 2-C, 2013, 93-108. doi: 10.21608/absb.2013.6422
HAGAGGI, N., HEZAYEN, F., ABDULRAOUF, U. (2013). 'PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T', EKB Journal Management System, 24(Issue 2-C), pp. 93-108. doi: 10.21608/absb.2013.6422
HAGAGGI, N., HEZAYEN, F., ABDULRAOUF, U. PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T. EKB Journal Management System, 2013; 24(Issue 2-C): 93-108. doi: 10.21608/absb.2013.6422

PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T

Al-Azhar Bulletin of Science
Article 18, Volume 24, Issue 2-C, December 2013, Page 93-108  XML PDF (279.94 K)
Document Type: Original Article
DOI: 10.21608/absb.2013.6422
View on SCiNiTO View on SCiNiTO
Authors
NOURA HAGAGGI1; FRANCIS F. HEZAYEN1; USAMA M. ABDULRAOUF* 2
1Botany Department, Faculty of Science, Aswan University, Aswan, Egypt
2Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt
Abstract
The extremely halophilic archaeon, Natrialba aegyptiaca strain 40T, which was isolated from a salty soil close to Aswan, Egypt is able to produce a halophilic extracellular, raw starch-digesting amylase. Optimization of medium components and culture conditions to enhance amylase production was investigated. Maximum production of this amylase was achieved in a medium contained (%, w/v): NaCl, 15; KCl, 1.5; MgSO4.7H2O, 0.1 and soluble starch, 0.05 at a pH range between 6-8 and a temperature range between 45-47 oC after incubation period of 144 hr. under static conditions and 48 hr. with shaking at 150 rpm. The enzyme could efficiently hydrolyze raw starches from different plant sources.
Keywords
halophilic; Natrialba aegyptiaca; amylase; production; Optimization; raw starches
Statistics
Article View: 198
PDF Download: 311