SPECTROFLUORIMETRIC METHOD FOR DETERMINATION OF SOME OXICAMS USING POTASSIUM BROMATE | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 2, Volume 31, Issue 2, December 2008, Page 169-181 PDF (274.27 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2008.64222 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Hanaa M. Abdel-Wadood | ||||
| Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt, 71526 | ||||
| Abstract | ||||
| A sensitive and selective spectrofluorimetric method has been developed for the determination of some non-steroidal antiinflammatory oxicams derivatives namely: tenoxicam (Tx), piroxicam (Px) and lornoxicam (Lx) after their complete oxidative acidic hydrolysis to 2-aminopyridine. The hydrolytic product 2- aminopyridine exhibits fluorescence emission at 365 nm (excitation at 305 nm). The optimal conditions of the reaction were investigated. The method was found to be linear in the ranges of (0.015-0.500 μg/ml) for Tx (0.006-0.300 μg/ml) for Px and (0.060- 0.200 μg/ml) for Lx. The suggested method was successively applied for the determination of the studied drugs in different dosage forms with a recovery percentages ranged 96.82-102.79 ± 0.614-2.578. The method was also applied for the determination of the drugs in spiked urine with a recovery percentages ranged 80.51-105.35 ± 1.067-5.338. The validity of the method was assessed according to USP guidelines. Statistical analysis of the results reveled high accuracy and good precision. | ||||
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