UTILITY OF CERTAIN SPECTROFLUORIMETRIC METHODS FOR ANALYSIS OF TWO PHARMACEUTICAL BINARY MIXTURES | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 3, Volume 31, Issue 2, December 2008, Page 183-195 PDF (269.37 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2008.64223 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Kamla M. Emara; Hanaa M. Abdel-Wadood* ; Marwa F. Bakr | ||||
| Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt | ||||
| Abstract | ||||
| Simple and very sensitive spectrofluorimetric methods were developed for determination of adrenaline (I)–procaine hydrochloride (II) mixture and salbutamol sulfate (III) – guaifenesin (IV) mixture. Adrenaline (I) in the first mixture was determined by coupling with 5-diazo-1,2,4-triazolo-3-carboxylic acid (DTCA) reagent in alkaline medium forming fluorigenic product which can be measured at 340 nm (ex. 245 nm), while procaine hydrochloride (II) gave no fluorescence. Salbutamol sulfate (III) was analyzed by reaction with ethyl acetoacetate (EAA) forming coumarin derivative, which can be measured at 320 nm (ex. 280 nm). Guaifenesin (IV), the second drug in mixture has a considerable native fluorescence in methanol was measured at 310 nm (ex. 230 nm). All variables affecting reaction conditions were optimized. Linear correlations were obtained over the range of 19-100, 37-400 and 22-150 ng/ml for (I), (III) and (IV), respectively. The proposed methods were successfully applied for the analysis of the studied drugs in their pure and commercial dosage forms and the obtained results were in good agreement with those obtained from the reported methods; no significant difference in the accuracy and precision as revealed by the accepted values of t- and F-tests, respectively. | ||||
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