BIOCHEMICAL STUDIES ON VERTICAL TRANSMISSION OF A TOXOPLASMA CIRCULATING ANTIGEN
A.M, A., M.A., E., AM, E. (2009). BIOCHEMICAL STUDIES ON VERTICAL TRANSMISSION OF A TOXOPLASMA CIRCULATING ANTIGEN. EKB Journal Management System, 5(1), 1-15. doi: 10.21608/blj.2008.64231
Attallah A.M; EL-Mouayed M.A.; El-Waseef AM. "BIOCHEMICAL STUDIES ON VERTICAL TRANSMISSION OF A TOXOPLASMA CIRCULATING ANTIGEN". EKB Journal Management System, 5, 1, 2009, 1-15. doi: 10.21608/blj.2008.64231
A.M, A., M.A., E., AM, E. (2009). 'BIOCHEMICAL STUDIES ON VERTICAL TRANSMISSION OF A TOXOPLASMA CIRCULATING ANTIGEN', EKB Journal Management System, 5(1), pp. 1-15. doi: 10.21608/blj.2008.64231
A.M, A., M.A., E., AM, E. BIOCHEMICAL STUDIES ON VERTICAL TRANSMISSION OF A TOXOPLASMA CIRCULATING ANTIGEN. EKB Journal Management System, 2009; 5(1): 1-15. doi: 10.21608/blj.2008.64231

BIOCHEMICAL STUDIES ON VERTICAL TRANSMISSION OF A TOXOPLASMA CIRCULATING ANTIGEN

Biochemistry Letters
Article 1, Volume 5, Issue 1, 2009, Page 1-15  XML PDF (310.79 K)
Document Type: Original Article
DOI: 10.21608/blj.2008.64231
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Authors
Attallah A.M1; EL-Mouayed M.A.2; El-Waseef AM3
1R&D Dept., Biotechnology Research Center, New Damietta City, Egypt
2R&D Dept., Biotechnology Research Center, NewDamietta City, Egypt
3Chemistry Dept., Faculty of Science,Mansoura Universty, Egypt
Abstract
The detection of toxoplasma-specific antibodies is the primary diagnostic method to determine infection with toxoplasma in pregnant women. The present study aimed to evaluate the vertical transmission of anti-Toxoplasma gondii antibodies (IgM, IgG). ELISA is one of the easiest tests and most helpful technique in the diagnosis of toxoplasmosis. The 36 kDa toxoplasma gondii- (T.gondi) circulating antigen was isolated and purified from serumof toxoplasma infected mothers and umbilical cord samples of their neonates by electroelution. This antigen was used to detect the anti- T.gondii IgM and IgG antibodies in 95 serum samples from pregnant mothers and 95 umbilical cord samples collected from the umbilical cord of their neonates. Total anti-T.gondii IgM antibodies were detected in 32 (33.7%) serum samples with vertical transmission rate (9.4%). Total anti-T.gondii IgG antibodies were detected in 26 (27.4%) serum samples with vertical transmission rate (53.8%). The serodiagnosis of toxoplasmosis based on the detection of antibodies is not useful for the detection of toxoplasma vertical transmission. However, T.gondii circulating antigen was detected in 55 (58%) serum samples with vertical transmission rate (81.8%) in respect to antigen positive. Therefore, the detection of T.gondii circulating antigen in umbilical cord serum is considered as a good evidence for vertical transmission of this antigen.
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