IN VIVO AND IN VITRO EXPRESSIONS OF CCR1 AND CCR5 IN PATIENTS WITH ACTIVE SCHISTOSOMIASIS AND CHRONIC LIVER DISEASE | ||||
| Biochemistry Letters | ||||
| Article 3, Volume 7, Issue 1, 2011, Page 45-68 PDF (497.91 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/blj.2010.64242 | ||||
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| Authors | ||||
| Samir A. El-Masry1; Mona S. Gouida2; Khalid M. El-azab3; Mahmoud E Nasr4 | ||||
| 1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menufyia University, Sadat City, Egypt | ||||
| 2Flow Cytometry and Genetic Units, Mansoura Children Hospital, Mansoura University, Egypt. | ||||
| 3Medical Analysis Specialist, Egypt. | ||||
| 4Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menufyia University, Sadat City, Egypt. | ||||
| Abstract | ||||
| The CCL3 receptors, CCR1 and CCR5, are expressed in a series of cell types. The aim of the present study was to demonstrate, in vitro and in vivo, expressions of CCR1 and CCR5 on peripheral blood mononuclear cells (PBMCs) from healthy individuals and Schistosoma mansoni (S. mansoni) infected patients with chronic liver diseases (CLD). PBMCs were isolated from the blood of 55 individuals divided into four groups; Group I: thirty one patients with CLD; group II: six patients with active S. mansoni infection, diagnosed by parasitological examination; group III: eight healthy individuals, non-infected with both schistosomiasis or viral infections, followed by treatment with soluble S. mansoni eggs antigens (SEA) after short term cells culture, for in vitro studies and group IV: ten healthy individuals, non-infected with both schistosomiasis or viral infections and served as a negative control group. All PBMCs from the studied groups were stained with monoclonal antibodies against CD4, CD8, CCR1 and CCR5, then detected by a flow cytometry technique. In-vitro study revealed that CCR1 and CCR5 expressions in SEA treated short term culture of PBMCs were not significantly lower than healthy controls (P=0.56 and 0.298, respectively). Also, In-vivo study, in active schistosomiasis patients, the decrement were not significant (P=0.313 and 0.093, respectively) compared with a healthy control group. While in CLD patients there was an increment in expression of CCR1 with significant value (p=0.014), as well as in CCR5 but the increment were not significant (P=0.099). Patients with active schistosomiasis showed lower CD4 and higher CD8 T-cells count compared with healthy controls. The expressions of CD4 and CD8 T-cells were not significant (P= 0.368, 0.875, respectively). CCR1 and CCR5 expressions may play a role in recruitment of lymphocytes to the CLD patients. CD4 was down regulated and CD8 T cells were up regulated in PBMCs of active schistosomiasis patients. | ||||
| Keywords | ||||
| CCR1; CCR5; Schistosoma mansoni; Chronic liver diseases; active schistosomiasis; HCV; SEA; chemokine receptors | ||||
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