ISOLATION, IDENTIFICATION, AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM RETROPHARYNGEAL LYMPH NODE OF SHEEP IN ALMONOFIYAH, EGYPT
BAKHIET, E., MOHAMED, M., MONTASSER, A., ABDUL-RAOUF, U. (2013). ISOLATION, IDENTIFICATION, AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM RETROPHARYNGEAL LYMPH NODE OF SHEEP IN ALMONOFIYAH, EGYPT. EKB Journal Management System, 24(Issue 2-C), 133-148. doi: 10.21608/absb.2013.6425
ELSAYED K. BAKHIET; MOHAMED O. MOHAMED; ABDEL-KHALECK MONTASSER; USAMA M. ABDUL-RAOUF. "ISOLATION, IDENTIFICATION, AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM RETROPHARYNGEAL LYMPH NODE OF SHEEP IN ALMONOFIYAH, EGYPT". EKB Journal Management System, 24, Issue 2-C, 2013, 133-148. doi: 10.21608/absb.2013.6425
BAKHIET, E., MOHAMED, M., MONTASSER, A., ABDUL-RAOUF, U. (2013). 'ISOLATION, IDENTIFICATION, AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM RETROPHARYNGEAL LYMPH NODE OF SHEEP IN ALMONOFIYAH, EGYPT', EKB Journal Management System, 24(Issue 2-C), pp. 133-148. doi: 10.21608/absb.2013.6425
BAKHIET, E., MOHAMED, M., MONTASSER, A., ABDUL-RAOUF, U. ISOLATION, IDENTIFICATION, AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM RETROPHARYNGEAL LYMPH NODE OF SHEEP IN ALMONOFIYAH, EGYPT. EKB Journal Management System, 2013; 24(Issue 2-C): 133-148. doi: 10.21608/absb.2013.6425

ISOLATION, IDENTIFICATION, AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM RETROPHARYNGEAL LYMPH NODE OF SHEEP IN ALMONOFIYAH, EGYPT

Al-Azhar Bulletin of Science
Article 21, Volume 24, Issue 2-C, December 2013, Page 133-148  XML PDF (368.71 K)
Document Type: Original Article
DOI: 10.21608/absb.2013.6425
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Authors
ELSAYED K. BAKHIET* 1; MOHAMED O. MOHAMED1; ABDEL-KHALECK MONTASSER2; USAMA M. ABDUL-RAOUF3
1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt
2Department of Brucellosis Research, Animal Health Research Institute, Dokki, Giza, Egypt
3Botany and Microbiology Department, faculty of Science, Al-Azhar University, Assiut, Egypt
Abstract
Brucellosis is economically important infection in Egypt, and the brucellosis causative agent has not been genetically characterized in detail. In this study Brucella melitensis bv.3 was isolated and characterized from retropharyngeal lymph node and other lymphoid organs of sheep and goats from Almonofiyah Governorate & Albehera Governorate, Egypt, and to characterize one of the Br. melitensis isolates by 16S rRNA gene sequencing and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR_RFLP) of outer membrane protein (omp2a) gene. Br. melitensis was isolated and identified from 17 out of 47 (36.2%) samples from lymphoid organs of sheep& goats. The 16S rRNA gene of one of the Br. melitensis isolate was sequenced and identical as Br. melitensis bv.3 strain (Gene Bank accession No. KF177277.1). The genetic characterization of Br. melitensis in this study can be used for epidemiological studies,   control of ovine brucellosis. In our knowhow this is the first report of the16S rRNA gene sequence of Brucella in Egypt.                                       
Keywords
Brucella melitensis; 16s rRNA gene; Outer membrane protein (omp2a) gene
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