TELOMERASE AS A TUMOR MARKER IN HEPATOCELLULAR CARCINOMA | ||||
| Biochemistry Letters | ||||
| Article 3, Volume 8, Issue 1, 2012, Page 43-63 PDF (378.17 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/blj.2012.64254 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Wisam Abd El-Moaty Kolab | ||||
| Faculty of Science, El-Markab University, Libia | ||||
| Abstract | ||||
| This study was conducted on 25 healthy controls, 25 cirrhotic patients and 30 patients with HCC. All patients had been diagnosed with HCV-associated chronic liver disease. Methods: Serum PIVKAII and AFP were measured by enzyme linked immunosorbent assay (ELISA), while telomerase activity in peripheral blood was estimated by polymerase chain reaction- enzyme- linked immunosorbent assay (PCR- ELISA method). Results: Mean telomerase activity, PIVKAII and AFP levels were significantly higher in HCC patients as compared to both cirrhotic patients and controls, also a significant elevation in cirrhotic patients were found as compared to controls. Positive correlation was found between telomerase activity and size of hepatic focal lesions. Also, a positive correlation was found between both telomerase activity and PIVIKA-II and between the pathological grades of HCC. In HCC the sensitivity/specificity (88.2/79.6) of telomerase activity was much higher than both PIVIKA-II (80.5/69.3) and AFP (72.6/61.5). Conclusion: The usefulness of telomerase activity assay in HCC diagnosis and it's superiority to other tumor marker were recorded. Therefore, telomerase activity is a novel, available detector and prognostic marker for HCC diagnosis. | ||||
| Keywords | ||||
| Hepatocellular carcinoma; telomerase; PIVKA-II; telomerase PCR ELISA | ||||
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