RELATIONSHIP BETWEEN THE TUMOR NECROSIS FACTOR-Α AND ITS RECEPTOR GENE POLYMORPHISM AND TNF-Α PLASMA LEVELS IN EGYPTIAN RHEUMATOID ARTHRITIS PATIENTS | ||||
Biochemistry Letters | ||||
Article 11, Volume 8, Issue 1, 2012, Page 139-164 PDF (652.41 K) | ||||
DOI: 10.21608/blj.2012.64274 | ||||
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Authors | ||||
Yousri M. Hussein1; Samir Ab. Nassar2; Nahla H. El Shaer2 | ||||
1Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt and Head of Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Taif, KSA . | ||||
2Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt. | ||||
Abstract | ||||
Rheumatoid arthritis (RA) it is a model of multigenic inflammatory disorder in which tumor necrosis factor-α (TNF-α) plays an important role. Genetic factors may be implicated in the susceptibility to disease initiation .Objective: The aim of this study is to elucidate putative association between the −308 G/A polymorphism in the promoter region of the TNF-α gene and 196M/R polymorphism of TNFRII genes and susceptibility to disease onset of RA. Methods: A total of 160 RA patients and a control group of 40 healthy subjects were available for the study. All patients fulfilled the American College of Rheumatology revised criteria for RA, Single-nucleotide polymorphisms (SNPs) at position −308 of TNF-α and 196 of TNFRII genes were determined using restriction fragment length polymorphism–polymerase chain reaction (PCR–RFLP). The A allele of the −308 promoter polymorphism was more prevalent among the patients. 308GA genotype of TNF-α is associated with a significantly higher serum TNF-α level. TNFRII 196R allele more prevalent among the patients .Our findings suggest that the A allele of TNF-α and TNFRII 196M/R polymorphism are associated with RA susceptibility. | ||||
Keywords | ||||
Rheumatoid arthritis (RA); TNF-α; TNFRII; Gene polymorphism | ||||
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