INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS | ||||
| Biochemistry Letters | ||||
| Article 8, Volume 6, Issue 1, 2010, Page 106-112 PDF (477.99 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/blj.2010.64435 | ||||
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| Authors | ||||
| S. Eljaafari; A:B. El-Fighi; I.M. fahelbum | ||||
| Department of biochemistry faculty of medicine Alfateh University | ||||
| Abstract | ||||
| Bovine and rat prolactin gene promoter function by glucocorticoids. It was of interest, therefore to examine the possible inhibition by glucocorticoids of pit l induced activation of transcription from the 3'- rPrl promoter mutant generated for this study. The studies were carried out by co-transfection into glucocorticoids receptor (GR) deficient CV-1 cells both pin1 and (GR) expression vectors and the reporter CAT plasmids with upstream rPrl promoter fragments. The effect of glucocorticoids was examined by the Addition of dexamethasone (10-6 M) to the cultures immediately after transfection. In these studies glucocorticoids receptor (GR) deficient CV-l cells were used and the (GR) was expressed from an expression vector parker et al. (1994). However, in the case of prolactin promoter fragments (eg, - 1960/+ 38, -44/ -423, -190/-423 and – 75 /+ 38) decreased in pitl induced DAT gene expression was seen when the (GR) was expressed but it was evident even in the absence of glucocorticoids. | ||||
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