SPECTROPHOTOMETRIC ANALYSIS OF CEFEPIME THROUGH ITS Hg(I) COMPLEX | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 5, Volume 35, Issue 1, June 2012, Page 55-65 PDF (288.15 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2012.64602 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nawal A. El-Rabbat1; Hanaa M. Abdel-Wadood1; Mohammed Sayed2; Heba S. Mousa3 | ||||
| 1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||||
| 2Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt | ||||
| 3Drug Research Center, Assiut University, Assiut, Egypt | ||||
| Abstract | ||||
| A spectrophotometric procedure was developed for determination of cefepime depending on the complexation of the drug with Hg2(NO3)2 in acid medium (pH 3.5) and measuring the absorbance at 263 nm. Different variables affecting the reaction were studied and optimized. Under the optimized conditions, linear relationship with good correlation coefficient (0.999) was found between the absorbance and the concentration in the range of 3.65-40 µg ml-1. The limits of detection and quantitation were 1.20 and 3.65 µg ml-1 respectively. The stoichiometry of the reaction was studied using Yoe and Jones method and was found to be 1:2 ratio for cefepime : Hg (I). The method was successfully applied for determination of cefepime in its vial with average percentage recovery of 98.95±1.079. The results were favorably compared with those of reference method. The IR study of the formed complex was done and different probabilities of the formed complexes were suggested. | ||||
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