Adverse Outcomes of Repeated Chlorpyrifos- Ethyl and Methyl Exposure in Rats: The Ameliorating Role of N-Acetylcysteine
Ezz El-Din, E., Osman, K., Ahmed, N., El-Seedy, A. (2019). Adverse Outcomes of Repeated Chlorpyrifos- Ethyl and Methyl Exposure in Rats: The Ameliorating Role of N-Acetylcysteine. EKB Journal Management System, 40(OCTOBER- DECEMBER), 618-628. doi: 10.21608/asejaiqjsae.2019.64903
Eslam Ezz El-Din; Khaled Osman; Nabila Ahmed; Ayman El-Seedy. "Adverse Outcomes of Repeated Chlorpyrifos- Ethyl and Methyl Exposure in Rats: The Ameliorating Role of N-Acetylcysteine". EKB Journal Management System, 40, OCTOBER- DECEMBER, 2019, 618-628. doi: 10.21608/asejaiqjsae.2019.64903
Ezz El-Din, E., Osman, K., Ahmed, N., El-Seedy, A. (2019). 'Adverse Outcomes of Repeated Chlorpyrifos- Ethyl and Methyl Exposure in Rats: The Ameliorating Role of N-Acetylcysteine', EKB Journal Management System, 40(OCTOBER- DECEMBER), pp. 618-628. doi: 10.21608/asejaiqjsae.2019.64903
Ezz El-Din, E., Osman, K., Ahmed, N., El-Seedy, A. Adverse Outcomes of Repeated Chlorpyrifos- Ethyl and Methyl Exposure in Rats: The Ameliorating Role of N-Acetylcysteine. EKB Journal Management System, 2019; 40(OCTOBER- DECEMBER): 618-628. doi: 10.21608/asejaiqjsae.2019.64903

Adverse Outcomes of Repeated Chlorpyrifos- Ethyl and Methyl Exposure in Rats: The Ameliorating Role of N-Acetylcysteine

Alexandria Science Exchange Journal
Article 7, Volume 40, OCTOBER- DECEMBER - Serial Number 4, December 2019, Page 618-628  XML PDF (542.95 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2019.64903
View on SCiNiTO View on SCiNiTO
Authors
Eslam Ezz El-Din1; Khaled Osman1; Nabila Ahmed* 1; Ayman El-Seedy* 2
1Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University;
2Department of Genetics, Faculty of Agriculture, Alexandria University, P.O Box 21545, Alexandria, Egypt
Abstract
The ameliorating effect of 150 mg/kg b.w. of N-acetylcysteine (NAC) against the oral administration of 7.88 (1/25 LD50) or 202.07 (1/10 LD50) mg/kg/day for 14 days of chlorpyrifos- ethyl (CPF-E) and chlorpyrifos-methyl (CPF-M), respectively, was investigated using neurobehavioral and biochemical markers for this toxicity. Neurobehavioral tests; open field test (OFT), hole-board test (HBT), light/dark box test (LDBT) and elevated plus maze (EPM) showed increase frequency of exploration, low level of anxiety and locomotor in rats treated with either CPE-E or CPF-M, while the co-administration of  NAC to treated rats attenuated neurobehavioral parameters. Biomarkers such as acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), paraoxonase (PON) and adenosine 5'-triphosphatase, (ATP-ase) showed declining in their activities, while calcium (Ca+2) levels in brain were increased. However, the administration of NAC following the intoxification of CPF-E or CPF-M attenuated the values of these biomarkers. It can be concluded that NAC can be used to ameliorate the toxicity of certain organophosphorus compounds such as CPF-E and CPF-M and considered it as a choice for the prevention and treatment of either CPF-E or CPF-M -induced toxicity.
Keywords
chlorpyrifos; rat; Neurobehavioral; biochemical; N-acetylcysteine; Attenuation
Statistics
Article View: 353
PDF Download: 482