Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin
Khafagy, A., Shourbagy, M., Fadel, H., Salama, S. (2014). Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin. EKB Journal Management System, 19(2), 103-115. doi: 10.21608/scvmj.2014.65326
Ahmed Khafagy; Mamdoh Shourbagy; Hanaa Fadel; Samah Salama. "Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin". EKB Journal Management System, 19, 2, 2014, 103-115. doi: 10.21608/scvmj.2014.65326
Khafagy, A., Shourbagy, M., Fadel, H., Salama, S. (2014). 'Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin', EKB Journal Management System, 19(2), pp. 103-115. doi: 10.21608/scvmj.2014.65326
Khafagy, A., Shourbagy, M., Fadel, H., Salama, S. Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin. EKB Journal Management System, 2014; 19(2): 103-115. doi: 10.21608/scvmj.2014.65326

Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin

Suez Canal Veterinary Medical Journal. SCVMJ
Article 9, Volume 19, Issue 2, December 2014, Page 103-115  XML PDF (701.84 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2014.65326
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Authors
Ahmed Khafagy1; Mamdoh Shourbagy2; Hanaa Fadel3; Samah Salama* 4
1Bacteriology, Immunology and Mycology department- Faculty of Veterinary Medicine- Suez Canal University
2Anaerobics unit, animal health research institute, Dokki, Giza;
3Department of animal hygiene, zoonosis & animal behavior-Faculty of Veterinary Medicine-Suez Canal University
4Faculty of Veterinary Medicine-Suez Canal University
Abstract
Foodstuffs of animal origin may present hazards, due to bacterial contamination. This study was conducted to determine Escherichia coli in some types of animal source foods (raw milk, raw beef meat and raw poultry meat). Bacteriological examination of total 246 raw food samples of animal origin, raw milk, (105); beef meat (31) and poultry meat (110) were collected from different localities in Ismailia City showed that 64/246 (26.01%) of samples were infected with E. coli. Serological identification of (10)  E. colistrains revealed that they were belonged to O111 polyvalent 1; O44  polyvalent 2 (2 strains for each); O78 polyvalent 4; O146 polyvalent 2; O26 polyvalent 1; O119 polyvalent 1; O86 polyvalent 1 and O18 polyvalent 3 (1 strain for each). E. coli strains showed high susceptible rate to Ciprofloxacin (CIP), (100%) and high resistance (100%) to Ampicillin (AMP), Metronidazole (MTZ), Amoxicillin (AML), and Vancomycin (VAS). Four strains of the isolated E. coli  were submitted to molecular studies for detection of the eaeA (protein intimin), iutA (encoding aerobactin) and iss (increased serum survival protein) genes, by using PCR technique.     
 
Keywords
Escherichia coli; Phenotype; genotype; food products
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