OXIDIZED DIPHENYLAMINE AS A SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF SOME PHARMACEUTICAL THIOLS AND THIOAMIDES
Emara, K., Refaat, I., Abdel-Wadood, H., Hassan, M. (2005). OXIDIZED DIPHENYLAMINE AS A SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF SOME PHARMACEUTICAL THIOLS AND THIOAMIDES. EKB Journal Management System, 28(2), 225-236. doi: 10.21608/bfsa.2005.65397
K. M. Emara; I. H. Refaat; H. M. Abdel-Wadood; M. G. Hassan. "OXIDIZED DIPHENYLAMINE AS A SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF SOME PHARMACEUTICAL THIOLS AND THIOAMIDES". EKB Journal Management System, 28, 2, 2005, 225-236. doi: 10.21608/bfsa.2005.65397
Emara, K., Refaat, I., Abdel-Wadood, H., Hassan, M. (2005). 'OXIDIZED DIPHENYLAMINE AS A SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF SOME PHARMACEUTICAL THIOLS AND THIOAMIDES', EKB Journal Management System, 28(2), pp. 225-236. doi: 10.21608/bfsa.2005.65397
Emara, K., Refaat, I., Abdel-Wadood, H., Hassan, M. OXIDIZED DIPHENYLAMINE AS A SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF SOME PHARMACEUTICAL THIOLS AND THIOAMIDES. EKB Journal Management System, 2005; 28(2): 225-236. doi: 10.21608/bfsa.2005.65397

OXIDIZED DIPHENYLAMINE AS A SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF SOME PHARMACEUTICAL THIOLS AND THIOAMIDES

Bulletin of Pharmaceutical Sciences Assiut University
Article 9, Volume 28, Issue 2, December 2005, Page 225-236  XML PDF (318.12 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2005.65397
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Authors
K. M. Emara1; I. H. Refaat* 1; H. M. Abdel-Wadood1; M. G. Hassan2
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt
2Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt
Abstract
Oxidized diphenylamine is newly utilized as a redox spectrophotometric reagent for the determination of six pharmaceutically important thiol and thioamide drugs named: acetylcystiene, captopril, carbimazole, propylthiouracil, thiopental sodium, and tiopronin. The method is based on measurement of the decrease in absorption intensity of the oxidized diphenylamine (diphenylbenzidine violet, λmax= 580 nm) reagent as a result of the reduction effect of the analysed drugs. This reagent was instantaneously prepared by the oxidation of diphenylamine using ferric sulphate in sulphuric acid medium. The molar ratio of the chromogen reagent was determined to be 2:1; diphenylamine : iron (III). The decrease in colour intensity was found to be quantitatively dependant on drug concentration. Experimental variables including reagent concentration, acid type and concentration, dilution solvent, reaction time, temperature and stability were studied and optimized. Validation parameters including linearity range, detection and quantitation limits, precision, selectivity and robustness were evaluated. The proposed method was found to be simple, sensitive and accurate one indicated by the studied validation parameters. Good recoveries (98.0  0.14 – 100%,  0.98) were obtained by the suggested method and it was applied for the determination of the studied drugs in many pharmaceutical dosage forms available in the local market. Good agreement, indicated by acceptable t- & F- tests, was found between results obtained by the suggested method and those obtained by the reported or pharmacopoeial methods.
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