ANTHRAQUINONES PRODUCTION IN AERIAL PARTS-DERIVED CALLUS OF RUBIA TINCTORUM L. | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 6, Volume 26, Issue 1, June 2003, Page 49-54 PDF (219.57 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2003.65467 | ||||
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| Authors | ||||
| A. M. A. Abd El-Mawla; E. Y. Backheet; N. A. El-Emary | ||||
| Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||||
| Abstract | ||||
| Callus cultures were developed from the aerial parts of Rubia tinctorum L. on MS medium containing 6-benzylaminopurine and 1-naphthaleneacetic acid. The changes in callus weight and anthraquinones content were followed between week 1 and 6. Anthraquinones content was monitored by HPLC. The linear increase in fresh weight was found to be parallel to the anthraquinones production. Effect of medium pH, incubation temperature and growth regulators on callus growth and anthraquinones content was studied and optimised. pH 7.5 and temperature of 30° were found optimum for the callus growth. 6-Benzylaminopurine (0.5 mg/L) and 1-naphthaleneacetic acid (2.0 mg/L) were found the most appropriate growth regulators. The callus ethanolic extract showed the same picture on TLC as that of the roots. The anthraquinones alizarin (1), lucidin--ethyl ether (2), 1-hydroxy-2-hydroxymethylantraquinone- 3-O- -glucoside (3) and lucidin primeveroside (4) in addition to other four minor anthraquinone spots were found to be produced by these cultures. The four isolated compounds (1-4) were identified by spectral analysis (UV, IR and MS) as well as comparison with authentic samples. Aerial parts, roots and the callus were analysed for their anthraquinones content. The anthraquinones content of callus extract was about 91% and eighteen fold of the roots and aerial parts extract respectively. | ||||
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