Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
Ibrahim, E., El Helw, H., Shafik, N., Mekhail, M. (2017). Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus. EKB Journal Management System, 2(1), 35-42. doi: 10.21608/javs.2017.65605
Ehab E. Ibrahim; Hamed A.H. El Helw; Nermen Guda Shafik; Manal Awad Mekhail. "Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus". EKB Journal Management System, 2, 1, 2017, 35-42. doi: 10.21608/javs.2017.65605
Ibrahim, E., El Helw, H., Shafik, N., Mekhail, M. (2017). 'Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus', EKB Journal Management System, 2(1), pp. 35-42. doi: 10.21608/javs.2017.65605
Ibrahim, E., El Helw, H., Shafik, N., Mekhail, M. Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus. EKB Journal Management System, 2017; 2(1): 35-42. doi: 10.21608/javs.2017.65605

Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus

Journal of Applied Veterinary Sciences
Article 5, Volume 2, Issue 1, October 2017, Page 35-42  XML PDF (314.06 K)
Document Type: Original Article
DOI: 10.21608/javs.2017.65605
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Authors
Ehab E. Ibrahim email orcid 1; Hamed A.H. El Helw2; Nermen Guda Shafik3; Manal Awad Mekhail4
1Department of Foot and Mouth Disease, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
2Veterinary Serum and Vaccine Research Institute, svri@idsc.gov.eg.
3Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Egypt
4Veterinary Serum and Vaccine Research Institute, Abbassia, Egypt
Abstract
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time.
Keywords
ELISA; FMD; Immunization; PCR; Horse radish peroxidase; Serotypes
References
ABD EL-KARIM, A.S. 2007. Comparative studies on foot and mouth disease vaccine containing different adjuvants in sheep. M.Vet.Sci.Thesis (Infectious Diseases), Fac.Vet.Med.Cairo Univ.
ABD EL-RAHMAN, A.O.; FARAG, M.A.; SAMIRA EL-KILANY; ALI, S.M. AND MANAL, ABO EL-YAZEID AND ZEIDAN, S.M. 2006. Isolation and identification of foot and mouth disease virus during an outbreak of 2006 in Egypt. J. Kafr El-Sheikh Vet. Med. 4 (1): 451-464
AIDAROS, H. A. 2002. Regional status and approaches to control and eradication of FMD in the Middle East and North Africa. Rev. Sci. tech. Off. Int. Epiz., 21 (3): 451-458.
BARNETT, P., PULLEN, L., WILLIAMS, L., DOEL, T.R., 1996. Assessment of Montanide ISA 25 and ISA 206, two commercially available oil adjuvant. Vaccine 14 (13), 1187-1198.
CROWTHER, J.R. AND ABU EL-ZEIN, E.M.E. 1979. Application of enzyme linked immunosorbent assay to the detection and identification of Foot and Mouth Disease viruses. J. Hyg., 83 (3): 513-519.
DAVALOS-PANTOJA, L., ORTEGA-VINUESA, J. L., BASTOS- GONZALEZ, D., HIDALGO-ALVAREZ, R. 2000. A comparative study between the adsorption of IgY and IgG on latex particles. J. Biomater. Sci. Polym Ed. 11: 657-673.
DEPA PM, DIMRI U, SHARMA MC, TIWARI R. 2012. Update on epidemiology and control of Foot and Mouth Disease - A menace to international trade and global animal enterprise, Vet World, 5(11): , 694-704.
EL-SHEHAWY L, AZAB AMH, MOSSAD W, EL-SAYED E, ISMAIL A, DEGHADY W, 2012. Real time RT-PCR assay for detection of different serotypes of FMDV in Egypt, Vet World, 5(12): 732-737, doi: 10.5455/vetworld.2012.732-737
FARAG, M.A.; AGGOUR, A.M. AND DAOUD, A.M. 2005. ELISA as a rapid method for detecting the correlation between the field isolates of foot and mouth disease and the current used vaccine strain in Egypt. J.Vet.Med.Giza, 53 (4): 949-955.
FARAG M A, EMAN M EL-GARF AND HIAM M FAKHRY, 2011. Emergency Vaccination Of Cattle Using Foot And Mouth Disease Vaccine. Zag. Vet. J. (ISSN. 1110-1458) Vol. 39 No. 2 pp.188-198.

GAMAL, W.M.; MOUNIR, M.I.; EL-HELOW, H. A. AND KHODEIR, M.H. 2015. Using of FMD antibody prepared in chicken egg yolk for emergency immunization of cattle. J. Egypt vet. Med. Assoc. 75, no.4 605-624.
HAMBLIN, C., BARNETT, I.T.R. AND CROWTHER, J.R.1986. A new enzyme linked immunosorbent assay (ELISA) for the detection of antibodies against FMD virus. II. Application. J. Immunol. Meth., 93: 123- 129.
HIAM, M. FAKHRY, KHOUDEIR M. AND ., HANAA A. MOSTAFA, 2013 .
Comparative studies on foot and mouth disease SAT 2 hyper immune sera prepared in different hosts conjugated with flourescein isothiocyanate and horse radish peroxidase . 6th Sci. Congr. of Egypt. Soc. For Anim. Manag. 27-31 August.,: 14 -23.
HOFFMANN, J. P. AND RICHTERICH, R. 1970. The elimination of turbidity in the determination of plasma proteins with Biuret reagent. Z. Klin, chem.. J. Klin. Biochem., 8:595-598.
HUDSON, L., AND HAY, F. C. 1989. Practical Immunology 3rd Ed. Blackwell Scientific Publications Oxford London. ISBN. 0-632-01491-1
IKEMORI, Y.; OHTA, M.; UNEDA, K.; I CATLO, FC. JR.; KUROKI, M.; YOKOYAND, H. AND KODANA, Y. 1997. Passive protection of neonatal calves against bovine coronavirus - induced diarrhea by administration of egg yolk or colostrum antibody powder. Vet Microbiol., 58 (2-4): 105-111.
KARLSSON, M., KOLLBERG, H., LARSSON, A., 2004. Chicken IgY: utilizing the evolutionary advantage. World's Poult. Sci. J. 60, 341.
LESLIE GA and CLEM LW. 1969. Phylogen of immunoglobulin structure and function. Immunoglobulins of the chicken. J Exp Med. 130:1337–52.
MANAL ABOEL-YAZED MUSTAFA AND MERVAT M.ALI. 2008. Preparation of anti-FMD virus type-A immuno globuline conjugated with horse radish peroxidase and fluorescein isothiocyanate for serological use. J. Egypt. Vet. Med. Assoc. 68(3) 2008, Arab vet.. Medical congress. (15-17) December 2008, pp.(129-139) .
MCCULLOUGH, K. K.; CROWTHER, J. R., AND BUTCHER, R. N. 1985. A liquid –phase ELISA and its use in the identification of epitopes of Foot and mouth disease virus antigen. J. Virol. Methods. 11: 329-338.
METTIAS, K.N.; EL - RASHIDY.; A. A.; EL-GHARHI, M.; HUSSIEN, N. N.; HASSEN, E. R.; SALEH, S. M. AND EL - DANAF, N. A. 1994. Studies on the protection of bovine neonats against diarrhea by vaccination with trivalent vaccine (Rota, Corona, K99) - Estimation of K99 antibodies invaccinated dams and their neonates. Zag. Vet. J., 22 (3): 137-142.
NEETA LONGJAM, DEB R, SARMAH AK, TAYO T, AWACHAT VB, SAXENA VK. 2011. A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional toMolecular Tools. Veterinary Medicine International Volume 2011,

Article ID 905768, 17 pages, doi:10.4061/2011/905768.
OIE. 2009. OIE Terrestrial Manual , Foot and Mouth Disease chapter 2.1.05
OIE. 2013. OIE/FAO Foot-and-Mouth Disease Reference Laboratory Network,Annual Report 2013 .
PARIDA SATYA. 2009. Vaccination against foot‑and‑mouth disease virus strategies and effectiveness Expert Rev. Vaccines 8(3), 347–365.
POLSON , A. , VON WECHMAR , M. B., AND VAN REGENMORTEL, M. H. 1980 . Isolation of viral IgY antibodies from yolks of immunized hens. Immunol. Commun. 9 : 475 – 493 .
RAI, A. AND LAHIRI, D.K. 1981. A micro-enzyme labeled immunosorbent assay (micro- ELISA) for the detection of Foot and Mouth Disease virus antigen and antibody. Acta. Virol., 25 (1): 49-52.
ROEDER, P. L. AND LE BLANC SMITH, P. M. 1987. Detection and typing of foot-and-mouth disease virus enzyme linked Immunosorbent assay: A sensitive, rapide and reliable technique for primary diagnosis. Res. Vet. Sci. 43: 225-232.
SAFY E. M .2011. Evaluation of emergency vaccination against Foot and Mouth disease using bivalent type A&O1. PhD, faculty of veterinary medicine, Benha University
SCHADE, R., HLINAK, A. 1996. Egg Yolk Antibodies. State of the Art and Future Prospects. ALTEX. 13:5-9.
SHAWKY M., ABD EL-ATY M., HIAM. M. FAKRY, HIND M. DAOUD, EHAB EL-SAYED I., WAEL MOSSAD G., SONIA A. RIZK, ABU-ELNAGA H., MOHAMED A. A., ABD EL-KREEM A. AND FAROUK E. M. 2013. Isolation and Molecular Characterization of Foot and Mouth Disease SAT2 Virus during Outbreak 2012 in Egypt. J Vet Adv 2013, 3(2): 60-68. DOI: 10.5455/jva.20130219104353.
SOCK HWEE TAN, ABIDALI MOHAMEDALI, AMIT KAPUR, LAURA LUKJANENKO, MARK S. BAKER .2012. A novel, cost-effective and efficient chicken egg IgY purification procedure. Journal of Immunological Methods 380 (2012) 73–76
TINI, M., JEWELL, U. R., CAMENISCH, G., CHILOV, D., GASSMANN, M. 2002. Generation and application of chicken egg-yolk antibodies. Comp. Biochem. Phys.131: 569-574.
XUAN H., LI Y., FANG, H. AND ZHENG, C. 2011. Establishment of persistent infection with foot and mouth disease virus in BHK21 cells. Virol. J., 8: 169

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