Human Platelet Alloantigens (HPA-3) Polymorphism in Sickle Cell Disease Patients with Vaso-Occlusive Crisis | ||||
| The Medical Journal of Cairo University | ||||
| Article 81, Volume 87, September, September 2019, Page 3387-3392 PDF (465.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2019.65635 | ||||
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| Authors | ||||
| IMAN R. EL-MAHGOUB, M.D.; AZZA A. ALI, M.D.; AMIRA S.A. FARHAN, M.Sc.; MONA K. EL-GHAMRAWY, M.D.; HEBATULLLA FAROUK, M.D.; SOHA ATEF, M.D.; GEHAN H. SHAHIN, M.D.; RANIA SOLIMAN, M.Sc. | ||||
| The Department of Clinical & Chemical Pathology, Faculty of Medicine Cairo University*, National Research Centre** and Pediatric Department, Faculty of Medicine Cairo University*** | ||||
| Abstract | ||||
| Abstract Background: Vaso-occlusive crisis (VOC) is a significant cause of morbidity and mortality in sickle cell disease (SCD) patients. As polymorphisms in human platelet antigens (HPA) exhibit a prothrombotic nature, we hypothesized that specific HPA polymorphisms could have a role in the pathogenesis of VOC in SCD. Aim of Study: This study investigated HPA-3 T2622G among Egyptian SCD patients. Patients and Methods: This study included 100 SCD patients and 50 controls. Patients were divided into, VOC group (n=60), and steady-state group (n=40). Genotyping was done using PCR-based Restriction Fragment Length Polymor-phism (RFLP) technique. Results: The HPA-3 mutant genotypes were significantly associated with SCD compared to controls (p=0.001), while no significant difference was observed between VOC and steady-state groups (p=0.169). Regarding the frequency of VOC episodes, the HPA-3 homozygous mutant genotypes showed significant differences (p=0.001). The HPA-3 mutant genotypes were significantly correlated with generalized type of VOC (p=0.006) and need for hospitalization (p=0.003). Regarding VOC complications, the HPA-3b/3b genotype was significantly associated with acute chest syndrome (p=0.008) and stroke (p=0.012). Conclusion: The HPA-3 T2622G is common among SCD patients. Although it is not a major determinant of vasculo-cclusion in SCD, it is significantly associated with VOC complications and may alter their outcome. | ||||
| Keywords | ||||
| Sickle cell disease; Vaso-occlusive crisis; HPA; PCR-RFLP | ||||
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