Human Platelet Alloantigen (HPA-5) Polymorphism in Sickle Cell Disease Patients with Vaso-Occlusive Crisis | ||||
| The Medical Journal of Cairo University | ||||
| Article 93, Volume 87, September, September 2019, Page 3501-3506 PDF (457.58 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2019.65647 | ||||
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| Authors | ||||
| IMAN R. EL-MAHGOUB, M.D.; AZZA A. ALI, M.D.; AMIRA S.A. FARHAN, M.Sc.; MONA K. EL-GHAMRAWY, M.D.; HEBATULLLA FAROUK, M.D.; SOHA ATEF, M.D.; GEHAN H. SHAHIN, M.D.; RANIA SOLIMAN, M.Sc. | ||||
| The Department of Clinical & Chemical Pathology, Faculty of Medicine, Cairo University*, National Research Centre** and Pediatric Department, Faculty of Medicine Cairo University*** | ||||
| Abstract | ||||
| Abstract Background: Vaso-occlusive crisis (VOC) is a significant cause of morbidity and mortality in sickle cell disease (SCD) patients. As polymorphisms in human platelet antigens (HPA) exhibit a prothrombotic nature, we hypothesized that specific HPA polymorphisms could have a role in the pathogenesis of VOC in SCD. Aim of Study: This study investigated HPA-5 G1648A polymorphism among Egyptian SCD patients. Patients and Methods: This study included 100 SCD patients and 50 controls. Patients were divided into, VOC group (n=60), and steady-state group (n=40). Genotyping was done using PCR-based Restriction Fragment Length Polymor-phism (RFLP) technique. Results: The HPA-5 mutant genotypes were significantly associated with SCD compared to controls (p=0.003), while no significant difference was observed between VOC and steady-state groups (p=0.179). Regarding the frequency of VOC episodes, the HPA-5 homozygous mutant genotypes showed significant differences (p=0.003). Regarding VOC complications, the HPA-5b/5b genotype was significantly associated with acute chest syndrome only (p=0.021). Conclusion: The HPA-5 G1648A polymorphism is common among SCD patients. Although neither of them is a major determinant of vasculo-cclusion in SCD, they are significantly associated with VOC complications and may alter their outcome. | ||||
| Keywords | ||||
| Sickle cell disease; Vaso-occlusive crisis; HPA; PCR-RFLP | ||||
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