Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique
Rabeh, M., El-Hawary, S., El-Tantawy, M., Abdelmohsen, U., Badr, W. (2020). Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique. EKB Journal Management System, 3(1), 40-44. doi: 10.21608/jabps.2019.20728.1062
Mohamed Rabeh; Seham Salah El-Din El-Hawary; Mona El-Mahdy El-Tantawy; Usama Ramadan Abdelmohsen; Wafaa Korany Badr. "Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique". EKB Journal Management System, 3, 1, 2020, 40-44. doi: 10.21608/jabps.2019.20728.1062
Rabeh, M., El-Hawary, S., El-Tantawy, M., Abdelmohsen, U., Badr, W. (2020). 'Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique', EKB Journal Management System, 3(1), pp. 40-44. doi: 10.21608/jabps.2019.20728.1062
Rabeh, M., El-Hawary, S., El-Tantawy, M., Abdelmohsen, U., Badr, W. Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique. EKB Journal Management System, 2020; 3(1): 40-44. doi: 10.21608/jabps.2019.20728.1062

Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique

Journal of advanced Biomedical and Pharmaceutical Sciences
Article 5, Volume 3, Issue 1, January 2020, Page 40-44  XML PDF (533.52 K)
Document Type: Original Article
DOI: 10.21608/jabps.2019.20728.1062
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Authors
Mohamed Rabeh email 1; Seham Salah El-Din El-Hawary2; Mona El-Mahdy El-Tantawy3; Usama Ramadan Abdelmohsenorcid 4; Wafaa Korany Badr3
1Department of Pharmacognosy, Faculty of pharmacy, Cairo University, Cairo 11562, Egypt.
2Department of Pharmacognosy, Faculty of pharmacy, Cairo University, Cairo 11562, Egypt.o, Egypt
3Department of Medicinal plants and natural products, National Organization of Drug Control and Research, Giza, Egypt.
4Department of Pharmacognosy, faculty of Pharmacy, Minia University, 61519 Minia, Egypt
Abstract
Opuntia is a large genus of succulent shrubs characterized by unique attractive flowers known as prickly pears by virtue of their characteristic edible fruits. They are used as laxative, diuretic, antipyretic, and anti-inflammatory.
This study aims to authenticate eight different Opuntia species, Opuntia brasilliensis (Willd.) Haw (O1), Opuntia dillenii (ker Gawl.) Haw. (O2), Opuntia dejecta Salm-Dyck (O3), Opuntia ficus indica (L.) Mill. (O4), Opuntia tomentosa Salm-Dyck (O5), Opuntia phaecantha Engelm. (O6), Opuntia leucotricha DC. (O7), Opuntia microdasys (Lehm.) Pleiff. (O8), growing in Egypt by using random amplified polymorphic DNA technique. Accurate identification of the eight Opuntia species is an urgent need to maintain herbal formulations potency and peculiarity.
Similarity coefficients of 40.0 - 67.6% classified Opuntia species into two main groups, one of them contains two species and the other one contains six species. Consequently, this technique helps the identification of these Opuntia species showing great morphological similarity.
Keywords
RAPD; Genetic diversity; DNA fingerprinting; Opuntia; Cactaceae
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