An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine
Farahat, S., Abdel Salam, R., Hadad, G., El-Gindy, A., Hassan, T. (2019). An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine. EKB Journal Management System, 3(1), 49-57. doi: 10.21608/rpbs.2019.66749
Sara Farahat; Randa A. Abdel Salam; Ghada Hadad; Alaa El-Gindy; Tamer Hassan. "An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine". EKB Journal Management System, 3, 1, 2019, 49-57. doi: 10.21608/rpbs.2019.66749
Farahat, S., Abdel Salam, R., Hadad, G., El-Gindy, A., Hassan, T. (2019). 'An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine', EKB Journal Management System, 3(1), pp. 49-57. doi: 10.21608/rpbs.2019.66749
Farahat, S., Abdel Salam, R., Hadad, G., El-Gindy, A., Hassan, T. An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine. EKB Journal Management System, 2019; 3(1): 49-57. doi: 10.21608/rpbs.2019.66749

An ecofriendly green liquid chromatographic method for simultaneous determination of Amoxicillin, Metronidazole and Ciprofloxacin; application to dosage form and human urine

Records of Pharmaceutical and Biomedical Sciences
Article 8, Volume 3, Issue 1, March 2019, Page 49-57  XML PDF (480.34 K)
Document Type: Original Article
DOI: 10.21608/rpbs.2019.66749
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Authors
Sara Farahat1; Randa A. Abdel Salamorcid 2; Ghada Hadad email orcid 2; Alaa El-Gindy2; Tamer Hassanorcid 3
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, El Arish, North Sinai, Egypt
2Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
3Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Abstract
Green liquid chromatographic method using cyano column and ethanol and 0.5% acetic acid in water (pH 2.8) as mobile phase in gradient mode has been developed for analysis of Amoxicillin, Metronidazole and Ciprofloxacin in human urine. Quantification was carried out using a diode-array UV detector, the detection wavelengths was 255nm. The retention times and detection limits for each antibiotic were 4.9 min and 0.5μg/mL for Amoxicillin, 6.035 min and 0.14 μg/mL for Metronidazole, 8.03 min and 3.07 μg/mL for Ciprofloxacin, respectively. The developed method was applied to examine the urinary excretion pattern of Amoxicillin, Metronidazole and Ciprofloxacin in healthy male volunteer after an oral administration of Metronidazole and Ciprofloxacin in their combination tablet (Ciprodiazole®) and combination tablet of Ciprofloxacin and Amoxicillin (Helicocin®). The developed HPLC method was successfully used for the analysis of the selected drugs in their dosage forms and human urine without interference from the excipients or urine matrix. The proposed method was rapid, specific, precise, accurate, environmentally friendly and suitable for bioequivalence and pharmacokinetic studies.
Keywords
Green; HPLC; ciprofloxacin; Metronidazole; amoxicillin; urinary excretion pattern
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