Susceptibility of Aminoglycoside Resistant Acinetobacter baumannii to Antibiotic Combinations | ||||
| Egyptian Journal of Microbiology | ||||
| Article 11, Volume 54, Issue 1, 2019, Page 137-147 PDF (1.37 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejm.2019.16809.1115 | ||||
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| Authors | ||||
Feriala Abo safe  1; Neven Salah2; Mohamed Rashed3; Sarah Saad1
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| 1Botany Department, College of Women for Arts, Science and Education, Ain Shams University Cairo, Egypt | ||||
| 2Microbiology Department, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt | ||||
| 3Microbiology Department, National Organization for Biological Control and Research, Cairo, Egypt | ||||
| Abstract | ||||
| ACINETOBACTER baumannii (A. baumannii ) is considered one of the predominant antibiotic resistance pathogens involved in hospital acquired infections worldwide problem. The study investigated the effect of antibiotic combination of β-lactams (ceftriaxone, cefixime, carpabenem and impenim) and aminoglycosides against 5 clinical isolates of A. baumannii multidrug resistant. Over one year, 250 bacterial isolates were collected from 5 Egyptian hospitals from various infection sites. Two hundred out of 250 bacterial isolates were identified as A. baumannii based on phenotypic and genotypic techniques. The susceptibility of two-hundred A. baumannii strains against 21 different antibiotics was studied. The results showed that the highest resistance was to Cephalosporins, group was 99% followed by Quinolones & Fluoroquinolone was 90, 5 followed by penicillin 87.5, then Sulfa drugs was 75.5 then Carbapenem was 73 and finally Aminoglycosides was 60.5%. The minimum inhibitory concentration (MICs) values of aminoglycosides resistant A. baumannii strains ranged from 32 to >512mg/ml and β-lactam group ranged from 16 to >512mg/ml. Forty-five combined microtitre checkerboards were performed against the 5 totally aminoglycoside resistant A. baumannii strains to assess the potential for combination therapy. Combination of aminoglycoside antibiotics with β-lactams showed synergy action in thirty- eight (84%) of total forty-five combinations. Synergy was achieved with 100%, with the following combinations GN/IMP, GN/CRO, GN/CFM, AK/CRO, AK/CFM, TOB/CRO and TOB/CFM. No synergism was observed with combination between amikacin and imipenem. | ||||
| Keywords | ||||
| Acinetobacter baumannii ; Aminoglycosides; β-lactams combination; Synergy | ||||
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