L-Carnitine protects kidney against ischemia reperfusion injury via suppression of expression of tubular kidney injury molecule (Kim-1) and Wnt proteins (Beta- Catenin) | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 3, Volume 40, Issue 1, January 2020, Page 32-45 PDF (631.21 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2019.14073.1023 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mohamed Adel  1; mohamed rami1; dalia shaheen2; Rehan Monir3; mamdouh basheir4; ahmed nabawy4; ahmed eladl5
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| 1Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt | ||||
| 2Department of Biochemistry, Faculty of Medicine, Mansoura University, Egypt | ||||
| 3Department of Biochemistry, Faculty of Medicine, Mansoura University, Egypt faculty of medicine, king khalid university | ||||
| 4Department of Anatomy, Faculty of Medicine, Mansoura University, Egypt | ||||
| 5Department of Pathology, Faculty of Medicine, Mansoura University, Egypt | ||||
| Abstract | ||||
| L-Carnitine is a unique nutritional supplement for athletes that has been recently studied as a potential treatment for certain renal disorders. However, its efficacy in renal ischemia reperfusion injuries has not been investigated. Rats were randomly assigned to one of five groups (n = 6/group):1, Control negative: received saline, 2,control positive I/R, rats received Sal and ischemia reperfusion at the end of fourth week and 3, L-Car +I/R group: same as Sal and ischemia reperfusion, but rats received L-Car (100 mg∕ kg) intraperitoneally daily for four weeks, 4, metformin +I/R group: same as Sal and ischemia reperfusion, but rats received oral metformin (300 mg/kg/day) daily for four weeks. 5, metformin +I/R group +L-Car: same as group 3, but rats received oral metformin (300 mg/kg/day) daily for four weeks. IR induced a significant increase in serum creatinine, serum K⁺ and serum KIM-1 peaked at 24 hours after I/R. KIM-1 and β -catenin expression is upregulated 168 hours after I/R. L-Car administration led to a significant decrease in serum creatinine in proportional to I/R group, serum K⁺ in proportional to I/R group, and a significant decrease in serum KIM-1 and Kim-1 expression. L-Car administration attenuated I/R-induced increase in oxidative stress marker MDA and increased antioxidant GSH activity. Moreover, the inducer of autophagy (Metformin) led to a significant decrease in serum creatinine, serum K⁺ and a significant decrease in serum Kim-1 and Kim-1 expression in renal tissue. Overall, our results suggest a potential therapeutic role of L-Car and metformin in I/R | ||||
| Keywords | ||||
| L-carnitine; Beta- Catenin; Kim-1 | ||||
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