Evaluation of The Analgesic Activity of Coenzyme Q10 Ointment in Mice | ||||
Journal of Applied Veterinary Sciences | ||||
Article 2, Volume 5, Issue 1, January 2020, Page 11-16 PDF (407.7 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/javs.2019.20868.1001 | ||||
View on SCiNiTO | ||||
Authors | ||||
Ibtisam T. Al-Jureisy1; Wael T. Al wattar2; Ghada A.Taqa 3 | ||||
1Ministry Of Health-Nineveh Health Directorate-Mosul-Iraq | ||||
2Faculty of Dyntistry, University of Musol, Iraq | ||||
3Professor in veterinary pharmacology and toxicology, College of Dentistry , Mosul university , Iraq | ||||
Abstract | ||||
The goal of the present study is to evaluate The Evaluation the effect of Analgesic Activity of Coenzyme Q10 ointment in mice. Materials and Methods: Fifteen healthy male albino mice were selected for this study. The animals were divided into three groups of five animals for each group. Group 1 served as a control and was applied vaseline ointment topically on fore and hind limb. Group 2 and 3 were applied topically Coenzyme Q10 ointment 4%, 8% respectively on fore and hind limb. The onset and duration of analgesic effect of Coenzyme Q10 ointment were evaluated in mice by utilizing a Hot-Plate test at 55±1˚C. Latency reaction time was recorded after 3min. and (10- 60 min) after ointment applied. The prolongation of latency times compared with the values of the control was used to express about analgesic effects of Coenzyme Q10 ointment and the percentage of Maximal Possible Effect (MPE) was calculated. Results: The Coenzyme Q10 ointment at concentrations (4%, 8%) produced analgesic effect in mice after 3 min, respectively in comparison with control. The percentage of maximum possible effect (MPE) was significantly increased in group treated with (4%,8%) Coenzyme Q10. Conclusions: The Coenzyme Q10 ointment have a good analgesic activity in mice after 3 minutes of topical application and prolong the duration of analgesia more than 40 minutes depending on concentration of Coenzyme Q10 ointment. | ||||
Keywords | ||||
Coenzyme Q10 ointment; Pain; Hot-plate test; analgesia in mice; Maximal Possible Effect | ||||
References | ||||
ACOSTA MJ, VAZQUEZ FONSECA L, DESBATS MA, CERQUA C1, ZORDAN R, TREVISSON E AND SALVIATI L.(2016).Coenzyme Q Biosynthesis in Health and Disease. Biochim Biophys Acta 1857(8): 1079-1085.
ALARCON E.(2002). A comparative study of the transdermal penetration of a series of non steroidal anti-inflmmatory drugs. J Pharm Sci 86 (24)503-308.
BHAGAVAN H. N. AND CHOPRA R. K. (2006).Coenzyme Q10: Absorption,Tissue uptake, Metabolism and Pharmacokinetics. Free Radic Res 40(5), 445-53.
COYLE JT AND PUTTFARCKEN P.(1993). Oxidative stress, glutamate, and neurodegenerative disorders. Science. 262(5134) : 689 -95.
CURTERO M, CARRIE H, MALIC U, ROMIC K, TASIOS B AND WU M. (2006). A comparision of frequently prescribed analgesics at the university of Toronto for postoperative pain following dental surgery. an evidancer-based study of the literature. Community Dentistry;1:1-12.
DAHL V AND READER JC.( 2000). Non-opioid postoperative analgesia. J Acta Anaesthesiol Scand;44:1191-1203.
DANIELS R AND KNIE U.(2002). Galenics of dermal product, vehicles, properties and drug release. JDDG 5:367-38.
DELEO JA AND COLBURN RW.(1995). The role of cytokines in nociception and chronic pain. In: Weinstein JN, Gordon SL, editors. Low Back Pain: A scientific and clinical overview. American Academy of Orthopaedic Surgeons pp. 163–185.
DINICOLANTONIO JJ, BHUTANI J, MCCARTY MF AND O''KEEFE JH.(2015).Coenzyme Q10 for The Treatment of Heart Failure:A review of The Literature. Open Heart; 2: e000326.
ENGELI S, FELDPAUSCH M, GORZELNIAK K, HARTWIG F, HEINTZE U AND JANKE J, ET AL.(2003). Association between adiponectin and mediators of inflammation in obese women. Diabetes. 52(4): 942–947.
ERNSTER L AND DALLNER G.(1995). Biochemical, physiological and medical aspects of ubiquinone function. Biochim Biophys Acta. 1271(1) : 195 -204.
FARSI F, MOHAMMADSHAHI M, ALAVINEJAD P, REZAZADEH A, ZAREI M AND ENGALI KA.(2016). Functions of Coenzyme Q10 Supplementation on Liver Enzymes, Markers of Systemic Inflammation, and Adipokines in Patients Affected by Nonalcoholic Fatty Liver Disease: A Double-Blind, Placebo-Controlled, Randomized Clinical Trial. J Am Coll Nutr.35(4): 346–353.
FERRANTE RJ, ANDREASSEN OA, DEDEOGLU A, FERRANTE KL, JENKINS BG AND HERSCH SM, ET AL.(2002). Therapeutic effects of coenzyme Q10 and remacemide in transgenic mouse models of Huntington''s disease. J Neurosci.22(5) : 1592 -9.
GHADA A. TAQA.(2014). Evaluation of antinociceptive activity of ketamine cream in rats. Human and Veterinary Medicine.
GHOSH MN.(1984). Fundamental of experimental pharmacology. Scientifibook Agency Calcutta, 2nd edition, p. 44-145.
GIUSTI G.(1997). The categorical status of determiners. In The New Comparative Syntax, New York: Longman Publishing Group, ed. L. Haegeman.pp. 95-123.
ILLUM L, FARRAJ NF AND DAVIS S.(1994). Chitosan as a novel nasal delivery system for peptide drugs. Pharm Res 11:1186-9.
JEANJEAN AP, MOUSSAOUI SM AND MALOTEAUX JM, ET AL.(1995). Interleukin-1 beta induces long-term increase of axonally transported opiate receptors and substance P. Neuroscience 68:151–157.
JENNIFER LEE, YEON SIK HONG, JEONG HEE JEONG, EUN JI YANG, JOO YEON JHUN, MI KYOUNG PARK, YOUNG OK JUNG, JUN KI MIN, HO YOUN KIM, SUNG HWAN PARK AND MI-LA CHO.(2013). Coenzyme Q10 Ameliorates Pain and Cartilage Degradation in a Rat Model of Osteoarthritis by Regulating Nitric Oxide and Inflammatory Cytokines.
KAPOOR M, MARTTEL-PELLETIER J, LAJEUNESSE D, PELLETIER JP AND FAHMI H. (2011). Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 7:33-42.
KREITLER S AND NIV D.(2007).Cognitive impairment in chronic pain. Pain Clinical Updates XV(4):1–4.
LEE BJ, HUANG YC, CHEN SJ AND LIN PT.(2012). Effects of coenzyme Q10 supplementation on inflammatory markers (high-sensitivity C-reactive protein, interleukin-6, and homocysteine) in patients with coronary artery disease. Nutrition. 28(7–8): 767–772.
MUSCOLI C, MOLLACE V, WHEATLEY J, MASINI E, NDENGELE M AND WANG ZQ, ET AL.(2004). Superoxide-mediated nitration of spinal manganese superoxide dismutase: a novel pathway in N-methyl-D-aspartate-mediated hyperalgesia. Pain. 111(1-2) : 96 -103.
NESAMI BN, MOZAFFARI-KHOSRAVI H, NAJARZADEH A AND SALEHIFAR E.(2015). The Effect of Coenzyme Q10 Supplementation on Pro-Inflammatory Factors and Adiponectin in Mildly Hypertensive Patients: A Randomized, Double-Blind, Placebo-Controlled Trial. Int J Vitam Nutr Res. 85(3–4): 156–164.
NICOL GD, LOPSHIRE JC AND PAFFORD CM.(1997). Tumor necrosis factor enhances the capsaicin sensitivity of rat sensory neurons. J Neurosci. 17:975–982.
NIKLOWITZ P, MENKE T, ANDLER W AND OKUN JG.(2004). Simultaneous analysis of coenzyme Q10 in plasma, erythrocytes and platelets:comparison of the antioxidant level in blood cells and their environment in healthy children and after oral supplementation in adults. Clin Chim Acta 342: 219-226.
OLIVIERI F, LAZZARINI R, BABINI L, PRATTICHIZZO F, RIPPO MR AND TIANO L,ET AL.(2013).Anti-inflammatory effect of ubiquinol-10 on young and senescent endothelial cells via miR-146a modulation. Free Radic Biol Med.63: 410–420.
RAMER MS, MURPHY PG AND RICHARDSON PM, ET AL.(1998). Spinal nerve lesion-induced mechanoallodynia and adrenergic sprouting in sensory ganglia are attenuated in interleukin-6 knockout mice. Pain 78:115–121.
SCHMELZER C, LINDNER I, RIMBACH G, NIKLOWITZ P, MENKE T AND DO¨RING F.(2008). Functions of coenzyme Q10 in inflammation and gene expression. Biofactors. 32(1–4): 179–183.
SCHMELZER C, LORENZ G, RIMBACH G AND DORING F.(2007). Influence of Coenzyme Q_{10} on release of pro-inflammatory chemokines in the human monocytic cell line THP-1. Biofactors.31(3–4): 211–217.
SCHWEIZER A, FEIGE U AND FONTANA A, ET AL.(1988). Interleukin-1 enhances pain reflexes. Mediation through increased prostaglandin E2 levels. Agents & Actions. 25:246–251.
SHEKELLE P, MORTON S AND HARDY ML.(2003). Effect of supplemental antioxidants vitamin C, vitamin E, and coenzyme Q10 for the prevention and treatment of cardiovascular disease. Evid Rep Technol Assess (Summ); 83: 1-3.
SINISCALCO D, FUCCIO C, GIORDANO C, FERRARACCIO F, PALAZZO E AND LUONGO L, ET AL.(2007). Role of reactive oxygen species and spinal cord apoptotic genes in the development of neuropathic pain. Pharmacol Res. 55(2) : 158 -66.
TSAI KL, HUANG YH, KAO CL, YANG DM AND LEE HC, ET AL. (2012). A novel mechanism of coenzyme Q10 protects against human endothelial cell from oxidative stree-induced injury by modulating NO-related pathway.J Nutr Biochem 23:458-468.
WAEL T. AL-WATTAR.(2011). The Efficacy of Triamcinalone in Controlling Pain and Swelling after Surgical Extraction of Teeth. Al-Rafidain Dent J. 11(1);32-36.
WANG ZQ, PORRECA F, CUZZOCREA S, GALEN K, LIGHTFOOT R AND MASINI E, ET AL.(2004).A newly identified role for superoxide in inflammatory pain. J Pharmacol Exp Ther.309(3): 869 -78.
ZHAI J, BO Y, LU Y, LIU C AND ZHANG L.(2017). Effects of Coenzyme Q10 on markers of inflammation: A systematic review and meta-analysis. PloS one. 12: e0170172.
ZHOU Y, WEI Y, WANG L, WANG X, DU X AND SUN Z, ET AL.(2011). Decreased adiponectin and increased inflammation expression in epicardial adipose tissue in coronary artery disease. Cardiovasc Diabetol. 10(1):1–9: 2. | ||||
Statistics Article View: 2,259 PDF Download: 680 |
||||