Determination of Volatile Constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi Fruits and Evaluation of Its Cytotoxic Activity | ||||
Journal of Advanced Pharmacy Research | ||||
Article 2, Volume 4, Issue 1, January 2020, Page 7-12 PDF (315.81 K) | ||||
Document Type: Research Article | ||||
DOI: 10.21608/aprh.2019.18355.1091 | ||||
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Authors | ||||
Nagwan Gabr 1; Suzan Mina 1; Jaky Talat2; Sherifa Fahmy3 | ||||
1Pharmacognosy Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt. | ||||
2Pharmacognosy Department, Faculty of Pharmacy, 6th October University | ||||
3Department of Pharmacognosy, Faculty of Pharmacy, 6th October University | ||||
Abstract | ||||
Objectives: Cucumis melo L. var. aegyptiacus cv. Ismailawi plant is a member of family Cucurbitaceae. This study aimed to identify volatile components of the pulp of the fruit and also to evaluate its cytotoxic activity. Methods: Automated rapid headspace solid phase micro extraction (SPME) was applied for extraction of the volatile components combined with gas chromatography coupled to mass spectrometry GC/MS for identification of the compounds responsible for the specific aroma of the fruits, MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) was used for the evaluation of the cytotoxic activity of both petroleum ether and defatted methanol extracts of the fruits’ pulp against 3 different tumor cell lines; colon (Caco-2), hepatic (HepG2) and cervix (HeLa) carcinoma cells. Results: Twenty volatile components were identified in the pulp of the fruits which were dominated by esters as the major class of components, with hexyl acetate representing the major constituent (23.07%). Petroleum ether extract of the pulp displayed mild cytotoxic activity against HepG2 cell line with IC50 of (31.6) µg/ml, all results were standardized against doxorubicinpositive control. Conclusion: This is the first report for the evaluation of the volatile constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi fruits which can serve as a base for its differentiation from other similar varieties in the same family. | ||||
Keywords | ||||
Cucumis melo; Cytotoxicity; Flavor; Volatile aroma | ||||
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