Potential of Pseudomonas putida F1 to manage Bean common mosaic virus of bean
Elsharkawy, M., El-Sawy, M. (2019). Potential of Pseudomonas putida F1 to manage Bean common mosaic virus of bean. EKB Journal Management System, 15(1), 201-210. doi: 10.21608/ajbs.2019.68228
Mohsen Mohamed Elsharkawy; Mohamed Mahmoud El-Sawy. "Potential of Pseudomonas putida F1 to manage Bean common mosaic virus of bean". EKB Journal Management System, 15, 1, 2019, 201-210. doi: 10.21608/ajbs.2019.68228
Elsharkawy, M., El-Sawy, M. (2019). 'Potential of Pseudomonas putida F1 to manage Bean common mosaic virus of bean', EKB Journal Management System, 15(1), pp. 201-210. doi: 10.21608/ajbs.2019.68228
Elsharkawy, M., El-Sawy, M. Potential of Pseudomonas putida F1 to manage Bean common mosaic virus of bean. EKB Journal Management System, 2019; 15(1): 201-210. doi: 10.21608/ajbs.2019.68228

Potential of Pseudomonas putida F1 to manage Bean common mosaic virus of bean

African Journal of Biological Sciences
Article 16, Volume 15, Issue 1, 2019, Page 201-210  XML PDF (636.36 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2019.68228
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Authors
Mohsen Mohamed Elsharkawy email 1; Mohamed Mahmoud El-Sawy2
1Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
2Plant Virology and Phytoplasma Research Dept., Plant Pathology Institutes, Agriculture Research Center, Giza, Egypt.
Abstract
The ability of the isolated rhizobacterial strain F1 of Pseudomonas putida to promote bean growth and resistance against Bean common mosaic virus (BCMV) was evaluated. Moreover, defense enzymes and the temporal expression profile of defense genes were assessed using quantitative real-time polymerase chain reaction (RT-qPCR) in bean plants. Bean plants treated with P. putida exhibited increased shoot and root dry weight relative to the control plants under greenhouse conditions. Similarly, the yield and yield components were increased in rhizobacterial treated bean plants under field conditions. Plants inoculated with P. putida showed the best inhibition effect of BCMV under greenhouse and field conditions. BCMV titer was significantly reduced in P. putida inoculated plants. Treatment with P. putida recorded the highest values of peroxidase and polyphenol oxidase enzymes after BCMV inoculation. The transcriptional profiles of PR1, PR2, PR3 and LOX genes were highly increased in P. putida treated plants. The obtained results in this study elucidate the potential of P. putida in controlling BCMV infection in bean plants as well as the involved mechanisms in disease resistance.
Keywords
Bean common mosaic virus; Pseudomonas putida; rhizobacteria; induced resistance; defense genes; RT-qPCR
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