INVESTIGATING THE EFFECT OF DIFFERENT ANIMAL VENOMS ON BREAST CANCER CELL LINES (MCF-7): IN VITRO STUDY
EL-FIKY, A., SHAHIN, H., MOHAMED, A., EL-RAYES, S. (2019). INVESTIGATING THE EFFECT OF DIFFERENT ANIMAL VENOMS ON BREAST CANCER CELL LINES (MCF-7): IN VITRO STUDY. EKB Journal Management System, 49(1), 28-38. doi: 10.21608/jesp.2019.68283
ABIR A. EL-FIKY; HADY S. SHAHIN; ALY F. MOHAMED; SALMA M. EL-RAYES. "INVESTIGATING THE EFFECT OF DIFFERENT ANIMAL VENOMS ON BREAST CANCER CELL LINES (MCF-7): IN VITRO STUDY". EKB Journal Management System, 49, 1, 2019, 28-38. doi: 10.21608/jesp.2019.68283
EL-FIKY, A., SHAHIN, H., MOHAMED, A., EL-RAYES, S. (2019). 'INVESTIGATING THE EFFECT OF DIFFERENT ANIMAL VENOMS ON BREAST CANCER CELL LINES (MCF-7): IN VITRO STUDY', EKB Journal Management System, 49(1), pp. 28-38. doi: 10.21608/jesp.2019.68283
EL-FIKY, A., SHAHIN, H., MOHAMED, A., EL-RAYES, S. INVESTIGATING THE EFFECT OF DIFFERENT ANIMAL VENOMS ON BREAST CANCER CELL LINES (MCF-7): IN VITRO STUDY. EKB Journal Management System, 2019; 49(1): 28-38. doi: 10.21608/jesp.2019.68283

INVESTIGATING THE EFFECT OF DIFFERENT ANIMAL VENOMS ON BREAST CANCER CELL LINES (MCF-7): IN VITRO STUDY

Journal of the Egyptian Society of Parasitology
Article 4, Volume 49, Issue 1, April 2019, Page 28-38  XML PDF (662.38 K)
Document Type: Original Article
DOI: 10.21608/jesp.2019.68283
View on SCiNiTO View on SCiNiTO
Authors
ABIR A. EL-FIKY1; HADY S. SHAHIN2; ALY F. MOHAMED1; SALMA M. EL-RAYES2
1VACSERA, Dokki, Giza, Egypt.
2Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Egypt.
Abstract
Traditional treatments of cancer include surgery, chemotherapy, radiotherapy, hormone therapy, and combination therapy which affect both cancer and normal cells. The present study investigated anticancer venom potentials of Montivipera xanthina and Leiurus quinquestriatus compared to the synthetic anti-estrogen drug (Tamoxifen®) on MCF-7 breast cancer cell line. Cytotoxicity of venoms was evaluated using MTT assay. Cell cycle arrest was done using propidium iodide DNA stained by Flow Cytometry. Apoptosis was determined using annexin V-FITC by Flow Cytometry. Gene expression analysis was done for Caspase3, HER2, ER, and Survivin using qRT-PCR 24hrs post treatment and histological examination. L. quinquestriatus & M. xanthina venoms and tamoxifen had IC50 at 2.5, 34.4 & 27.8μg/mL with MCF-7 cell line respectively. Cell cycle was ceased at G1/S phase of L. quinquestriatus and M. xanthina compared to tamoxifen. In gene expression analysis, venoms and tamoxifen exhibited apoptotic effect against MCF-7 cell lines by elevated expression of Caspase3 & depletion of HER2, ER, and Survivin. Histological examination showed apoptotic and slightly necrotic features in both venoms. Tamoxifen induced a number of apoptotic bodies and colonies of necrotic cells
Keywords
Venom; Montivipera xanthina; Leiurus quinquestriatus; MCF-7 breast cancer cell line; Cytotoxicity; apoptosis; Caspase-3; HER2; Er; survivin
Statistics
Article View: 211
PDF Download: 238