IMMUNO-MAGNETIC BEADS ELISA FOR DIAGNOSIS OF SCHISTOSOMA MANSONI INFECTION | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 7, Volume 49, Issue 1, April 2019, Page 51-59 PDF (267.01 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2019.68286 | ||||
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| Authors | ||||
| EMAN MOSTAFA H. MÉABED; ESSAM A. HASSAN | ||||
| Department of Parasitology, and Department of Tropical Medicine, Faculty of Medicine, Fayoum University, El Fayoum Postal Code 63513, Egypt. | ||||
| Abstract | ||||
| Diagnosis of Schistosoma mansoni infection is a challenge in low transmission regions. Serological diagnosis proved more sensitive and extensively used to detect anti- Schistosoma antibodies. This study evaluated the immunomagnetic beads-enzyme-linked immunosorbent assay (IMB-ELISA) for diagnosing S. mansoni infection in comparison with the commercial IHA, indirect ELISA and microscopy stool examination. A total of 100 subjects were divided into four groups (25 each). G1: cases actively passing S. mansoni eggs, G2: chronic cases of S. mansoni, G3: cases with other parasitosis and G4: normal healthy subjects. S. mansoni diagnostic rate was 25%, 46%, 48%, & 50% by parasitological, IHA, Indirect ELISA & IMB-ELISA, respectively. In G1, the four tests gave positivity rates of 100%, 88%, 96%, & 100%, respectively. In G2, the four tests gave 0%, 84%, 88% & 96%, respectively. Sensitivity of various tests were estimated in relation to total true positive S. mansoni cases (G1 & G2), and gave 50%, 86%, 92% & 98% for the applied tests, respectively. Cross-reactivity rates were estimated in relation to S.mansoni negative cases (G3 & G4). Specificity rates were 100%, 94%, 96% & 98%, respectively. | ||||
| Keywords | ||||
| Fayoum University Hospitals; Schistosoma mansoni; Magnetic beads; ELISA; IHA | ||||
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