Production of N-acetyl-D-glucosamine by submerged fermentation of the American cockroach’s (Periplaneta americana) Chitin
Salama, M., Ibrahim, N., Khaled, A., Abdul-AzizRadi, M. (2017). Production of N-acetyl-D-glucosamine by submerged fermentation of the American cockroach’s (Periplaneta americana) Chitin. EKB Journal Management System, 13(1), 91-98. doi: 10.21608/ajbs.2017.68636
Mahmoud Salama; Nevin Ibrahim; Amany Soliman Khaled; Magda Hassan Abdul-AzizRadi. "Production of N-acetyl-D-glucosamine by submerged fermentation of the American cockroach’s (Periplaneta americana) Chitin". EKB Journal Management System, 13, 1, 2017, 91-98. doi: 10.21608/ajbs.2017.68636
Salama, M., Ibrahim, N., Khaled, A., Abdul-AzizRadi, M. (2017). 'Production of N-acetyl-D-glucosamine by submerged fermentation of the American cockroach’s (Periplaneta americana) Chitin', EKB Journal Management System, 13(1), pp. 91-98. doi: 10.21608/ajbs.2017.68636
Salama, M., Ibrahim, N., Khaled, A., Abdul-AzizRadi, M. Production of N-acetyl-D-glucosamine by submerged fermentation of the American cockroach’s (Periplaneta americana) Chitin. EKB Journal Management System, 2017; 13(1): 91-98. doi: 10.21608/ajbs.2017.68636

Production of N-acetyl-D-glucosamine by submerged fermentation of the American cockroach’s (Periplaneta americana) Chitin

African Journal of Biological Sciences
Article 8, Volume 13, Issue 1, 2017, Page 91-98  XML PDF (302.31 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2017.68636
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Authors
Mahmoud Salama email 1; Nevin Ibrahim2; Amany Soliman Khaled1; Magda Hassan Abdul-AzizRadi1
1Entomology Department, Faculty of Science, Ain-Shams University
2Microbiology Department, Faculty of Science, Ain-Shams University
Abstract
N-acetyl-D-glucosamine (NAG) is a medical important molecule that linked linearly through β (1,4)– glycosidic linkages forming chitin. Chitin was isolated from the American cockroach’s dried exoskeletons and characterized by Infrared spectroscopy (IR), the data revealed that it was in α form with 108% degree of acetylation (DA). The isolated chitin in the colloidal form was used in submerged fermentation for NAG production using the chitinolytic entomopathogenic fungus Metarhizium anisopliae, AUMC2837which showed a great potentiality on chitin degradation and NAG production at starting pH rang 3-5. The maximum NAG production was estimated at initial pH value 4, where the fungus could produce 12.52±0.46 g/l NAG from 15 g/l colloidal chitin (approximately 83.46 % of the chitin degraded to NAG) after 96 hours of incubation. Such result highlighted Periplaneta Americana chitin to be a promising alternative source for NAG production. This is the first report on bio-production of NAG from American cockroach’s chitin by a native entomopathogenic fungus.
 
Keywords
chitin; N-acetylglucoseamine; fermentation; chitinases; chitinolytic activity; pH optimization
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