EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.
Khalil, A., El-Harairy, M., Abdel-Khalek, A., Al-Palani., Z. (2014). EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.. EKB Journal Management System, 5(4), 211-220. doi: 10.21608/jappmu.2014.69490
A. W. Khalil; M. A. El-Harairy; A. E. Abdel-Khalek; Z. M.R.K. Al-Palani.. "EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.". EKB Journal Management System, 5, 4, 2014, 211-220. doi: 10.21608/jappmu.2014.69490
Khalil, A., El-Harairy, M., Abdel-Khalek, A., Al-Palani., Z. (2014). 'EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.', EKB Journal Management System, 5(4), pp. 211-220. doi: 10.21608/jappmu.2014.69490
Khalil, A., El-Harairy, M., Abdel-Khalek, A., Al-Palani., Z. EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.. EKB Journal Management System, 2014; 5(4): 211-220. doi: 10.21608/jappmu.2014.69490

EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.

Journal of Animal and Poultry Production
Article 5, Volume 5, Issue 4, April 2014, Page 211-220  XML PDF (955.14 K)
Document Type: Original Article
DOI: 10.21608/jappmu.2014.69490
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Authors
A. W. Khalil; M. A. El-Harairy; A. E. Abdel-Khalek; Z. M.R.K. Al-Palani.
Animal Production Dept., Faculty of Agric., Mansoura Univ.
Abstract
The main objective of this study was to evaluate the effect of supplementing maturation media with, retinol (10 µmol/ml), ascorbic acid (100 µmol/ml) and α-tocopherol (100 µmol/ml), on cumulus cell expansion and in vitro nuclear maturation of buffalo oocytes. Oocytes were collected from ovaries taken from slaughterhouses. Only compact oocytes were used for in vitro maturation by TCM-199 medium supplemented with different vitamin levels for 22 h in CO2 incubator at 38.5 °C, 5% CO2 and 95% humidity.  Maturation rate was determined in terms of degree of expansion of cumulus cells and oocytes at metaphase-II (nuclear maturation). Results revealed that all supplements yielded higher percentages of oocytes showing full expansion of cumulus cells (77.2, 69.4 and 69.2% for tocopherol, ascorbic acid and retinol, respectively) as compared to control medium (60.2%), but the differences were not significant. Maturation rate in term of percentage of oocytes arrested at anaphase-I, telophase-I and metaphase-II was significantly higher for media supplemented with retinol, tocopherol and ascorbic acid, being 83.5, 82.2 and 81.94% as compared to 68.54% in control medium, respectively.
Conclusively, the present results may suggest that the supplementation of maturation medium (TCM-199) with retinol, ascorbic acid and α-tocopherol as a non enzymatic antioxidants had a beneficial effect on the cumulus cell expansion and nuclear maturation of buffalo oocytes. Retinol supplementation yielded the best results.  
 
Keywords
Buffalo oocytes; in vitro maturation; non enzymatic antioxidants
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