SPECTROPHOTOMETRIC DETERMINATION OF PIPERAZINE USING 3,5-DIBROMO-2-METHYL-p-BENZOQUINONE | ||||
Bulletin of Pharmaceutical Sciences Assiut University | ||||
Article 2, Volume 16, Issue 2, December 1993, Page 125-130 PDF (307.62 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bfsa.1993.70041 | ||||
View on SCiNiTO | ||||
Authors | ||||
H. A. Mohamed1; P. Y. Khashaba1; A. I. Mohamed1; A. A. El-Shorbagi2 | ||||
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||||
2Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||||
Abstract | ||||
A spectrophotometric method is developed for the quantitative determination of piperazine either in bulk drug or in pharmaceutical dosage forms. The method is based on the formation of vinylamine of this drug by interaction with acetaldehyde. The resulting enamine interacts with 3,5-dibromo, 2-methyl-p-benzoquinone (DEMQ) forming an intense blue colored aminovinylquinone which can be estimated spectrophotometrically at lmax642 nm. DEMQ is prepared according to a modified oxidation procedure by treatment of 2,4,6 tribromo-3-methylphenol with chromic oxide in a mixture of acetic acid and acetonitrile (9:1). Beer-Lambert's Law was adhered to over the range of 2-8, 2-9, and 2-10 mg.ml-1 for piperazine hydrate, tartrate, citrate and phosphate respectively. The optimum reaction conditions and the effect of all variables have been studied. The proposed method is simple, rapid, sensitive, accurate, precise and has been applied successfully for the analysis of some pharmaceutical formulations of piperazine including tablets, effervescent granules and syrup with good recoveries. Results obtained are in good agreement with those obtained by the official methods. | ||||
Statistics Article View: 127 PDF Download: 165 |
||||