A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA
Abdel-Khalek, M., Mahrous, M., Daabees, H., Beltagy, Y. (1993). A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA. EKB Journal Management System, 16(2), 163-168. doi: 10.21608/bfsa.1993.70203
M. M. Abdel-Khalek; M. S. Mahrous; H. G. Daabees; Y. A. Beltagy. "A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA". EKB Journal Management System, 16, 2, 1993, 163-168. doi: 10.21608/bfsa.1993.70203
Abdel-Khalek, M., Mahrous, M., Daabees, H., Beltagy, Y. (1993). 'A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA', EKB Journal Management System, 16(2), pp. 163-168. doi: 10.21608/bfsa.1993.70203
Abdel-Khalek, M., Mahrous, M., Daabees, H., Beltagy, Y. A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA. EKB Journal Management System, 1993; 16(2): 163-168. doi: 10.21608/bfsa.1993.70203

A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA

Bulletin of Pharmaceutical Sciences Assiut University
Article 7, Volume 16, Issue 2, December 1993, Page 163-168  XML PDF (270.42 K)
Document Type: Original Article
DOI: 10.21608/bfsa.1993.70203
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Authors
M. M. Abdel-Khalek; M. S. Mahrous; H. G. Daabees; Y. A. Beltagy
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria 21521, Egypt
Abstract
Rapid third derivative (D) spectrophotometric analysis procedures are described for the determination of aminoglutethimide and its N-acetyl metabolite in human plasma. The developed procedures minimize the mutual interference between these compounds and allow their determination without a previous extraction step. Aminoglutethimide and N-acetylaminoglutethimide are determined through the measurement of D3 amplitudes at 266 and 256 nm, respectively. Relative standard deviations for the assay of both compounds were less than 1.24%. Recoveries ranged from 97.2 to 99.8% for aminoglutethimide and from 97.0 to 99.4% for N-acetylaminoglutethimide.
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