A THIRD DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE IN VITRO DETERMINATION OF AMINOGLUTETHIMIDE AND ITS N-ACETYL METABOLITE IN HUMAN PLASMA | ||||
Bulletin of Pharmaceutical Sciences Assiut University | ||||
Article 7, Volume 16, Issue 2, December 1993, Page 163-168 PDF (270.42 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bfsa.1993.70203 | ||||
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Authors | ||||
M. M. Abdel-Khalek; M. S. Mahrous; H. G. Daabees; Y. A. Beltagy | ||||
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria 21521, Egypt | ||||
Abstract | ||||
Rapid third derivative (D) spectrophotometric analysis procedures are described for the determination of aminoglutethimide and its N-acetyl metabolite in human plasma. The developed procedures minimize the mutual interference between these compounds and allow their determination without a previous extraction step. Aminoglutethimide and N-acetylaminoglutethimide are determined through the measurement of D3 amplitudes at 266 and 256 nm, respectively. Relative standard deviations for the assay of both compounds were less than 1.24%. Recoveries ranged from 97.2 to 99.8% for aminoglutethimide and from 97.0 to 99.4% for N-acetylaminoglutethimide. | ||||
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